摘要
Objective: To observe the effect of acupuncture on blood oxygen concentration in the brain of rats with post-traumatic stress disorder (PTSD) based on functional near-infrared spectroscopy (fNIRS), thus to reveal the mechanisms of acupuncture in intervening the brain function of PTSD rats. Methods: Sixty Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, a grasping group, a paroxetine group and an acupuncture group, with 12 rats in each group. Except the blank group, rats in the other groups all received incarceration plus electric shock for 7 d to prepare the PTSD animal model. One hour before the stress model was established, rats in each group received the desig nated in tervention: rats in the blank group and the model group did not receive any intervention;rats in the grasping group received grasping and fixation;rats in the paroxetine group received paroxetine hydrochloride solution by intragastric administration;and rats in the acupuncture group received acupuncture. Six-day treatme nt was a course, with 2 courses of treatme nt con ducted for a total of 12 d. After the modeli ng, rats in each treatment group received intervention for 5 dz and the fNIRS system was used to collect and record the changes in the concentrations of oxygenated hemoglobin (HbO2), deoxygenated hemoglobin (d-Hb) and total hemoglobin (t-Hb) of the involved rat's brain regions, and also to assess the brain function. Results: Compared with the blank group, the con centrati on of HbO2 was significa ntly in creased, the concen trati on of d-Hb was significa ntly decreased, and the concen tration of t-Hb was sign ifica ntly in creased in the model group and the graspi ng group after the in tervention, and the differ en ces were statistically significant (all P<0.01). Compared with the model group, the concentrations of HbO2, d-Hb and t-Hb in the grasping group did not change significantly (all P>0.05). Compared with the grasping group, the concentration of HbC^was significantly decreased, the concentration o
目的:基于功能近红外光谱技术(fNIRS)观察针刺对创伤后应激障碍(PTSD)模型大鼠脑部血氧浓度的影响,揭示针刺干预PTSD的脑功能机制。方法:将60只Sprague-Dawley(SD)大鼠随机分为空白组、模型组、抓取组、帕罗西汀组和针刺组,每组12只。除空白组外,其余各组大鼠均以电击幽闭法复制PTSD动物模型,模型复制共7d。于应激造模前1h,各组接受相应干预:空白组和模型组不接受任何干预,抓取组接受抓取固定,帕罗西汀组接受盐酸帕罗西汀溶液灌胃,针刺组接受针刺治疗,6d为1个疗程,连续干预2个疗程,共计12d。造模结束后,各治疗组连续治疗5d后利用fNIRS系统采集并记录各组大鼠相关脑区组织氧合血红蛋白(HbO2)>脱氧血红蛋白(d-Hb)和总血红蛋白(t-Hb)浓度的变化情况,并进行脑功能评估。结果:干预结束后,与空白组比较,模型组和抓取组大鼠HbO2浓度显著升高,d-Hb浓度显著降低,t-Hb浓度显著升高,差异均具有统计学意义(均P<0.01);与模型组比较,抓取组大鼠HbO2.d-Hb和t-Hb浓度均无明显变化(均P>0.05);与抓取组比较,帕罗西汀组和针刺组大鼠HbO2浓度显著降低,d-Hb浓度显著升高,t-Hb浓度显著降低,差异均具有统计学意义(均P<0.05);帕罗西汀组与针刺组的HbO2> d-Hb和t-Hb浓度均无统计学差异(均P>0.05)。结论:针刺对PTSD模型大鼠脑区血氧浓度有良性的调节作用,这可能是针刺干预PTSD的重要脑功能机制。
基金
国家自然科学基金地区基金项目,No.81460744.
