摘要
目的初步探讨人健康牙周组织龈沟液中细胞因子的普遍水平,为确立诊断指标及后续研究提供正常标准范围参考。方法选择牙周组织健康者每人4～8颗后牙(第二前磨牙及第二磨牙),检查相关临床指标,测定龈沟液分泌量,并通过微量龈沟液炎症标志物组合的Luminex液相芯片多因子检测体系测定19种细胞因子表达水平,包括3种集落细胞刺激因子、7种白细胞介素、6种趋化性细胞因子、血管内皮细胞生长因子、肿瘤坏死因子-α以及干扰素-γ。结果检测到人健康牙周组织中209颗后牙(第二前磨牙103颗、第二磨牙106颗)龈沟液分泌量及所含19种细胞因子表达水平。结论初步确定人健康牙周组织龈沟液中与骨代谢密切相关的19种细胞因子的大致标准范围。
Objective To investigate the levels of the main cytokines from gingival crevicular fluid(GCF) in the healthy periodontal tissues. Methods GCF was sampled by filter paper strips from healthy people. The levels of 19 cytokines including 3 kinds of colonystimulating factor,7 kinds of interleukin,6 kinds of chemokine,vascular endothelial cell growth factor,tumor necrosis factor-α and interferon-γ were detected by Luminex. Results The contents of 19 kinds of cytokines were found in 209 posterior teeth of the healthy people. Conclusion The normal range of 19 kinds of cytokines in GCF from healthy periodontal tissues was initially identified.
作者
胡春晓
李茵
张振庭
HU Chun-xiao;LI Yin;ZHANG Zhen-ting(Capital Medical University School of Stomatology, Beijing 100050,China)
出处
《北京口腔医学》
CAS
2019年第2期84-88,共5页
Beijing Journal of Stomatology
基金
临床医学发展专项-"扬帆计划":临床技术创新项目(XMLX201402)
关键词
龈沟液
细胞因子
牙周组织健康
Gingival crevicular fluid
Cytokines
Healthy periodontal tissues
