摘要
目的:探讨β-连环蛋白(β-catenin)、G1/S-特异性周期蛋白-D1(Cyclin D1)、基质金属蛋白酶-9(MMP-9)和血管内皮生长因子(VEGF)与结肠癌病理转移的相关性。方法:选取海南医学院第二附属医院2016年1月—2017年12月收治的结肠癌患者120例,收集结肠癌组织、癌旁组织(距肿瘤2 cm)和结肠正常组织(距肿瘤5 cm),采用免疫组织化学SP法检测组织中β-catenin、Cyclin D1、MMP-9和VEGF表达情况。结果:β-catenin、Cyclin D1、MMP-9和VEGF在结肠癌组织内的阳性表达率分别为80.00%、71.67%、76.67%与88.33%,明显高于正常组织和癌旁组织(P<0.05)。β-catenin、Cyclin D1、MMP-9和VEGF阳性表达与结肠癌的淋巴结转移、浸润深度、分化程度、有无远处转移、Dukes分期和TNM分期具有明显的相关性(P<0.05);与患者年龄、性别、肿瘤部位和肿瘤直径无关(P>0.05)。结论:结肠癌组织中β-catenin、Cyclin D1、MMP-9和VEGF高表达与肿瘤转移密切相关,可作为预测结肠癌分期和淋巴结转移的分子生物学指标。
Objective:To investigate β-catenin,G1/S-specific cyclin-D1(Cyclin D1),matrix metalloprotein-9(MMP-9) and vascular endothelial growth factor(VEGF) Correlation between endothelial growth factor(VEGF) and pathological metastasis of colon cancer.Methods:120 patients with colon cancer admitted to the Second Affiliated Hospital of Hainan Medical College from January 2016 to December 2017 were enrolled.Colon cancer tissues,adjacent tissues (2 cm from cancer) and normal colon tissues (5 cm from cancer) were collected.Immunohistochemiscal.SP method was used to detect the positive expression of β-catenin,Cyclin D1,MMP-9 and VEGF in patients.Results:The positive expression rates of β-catenin,Cyclin D1,MMP-9 and VEGF in colon cancer tissues were 80.00%,71.67%,76.67% and 88.33%,respectively,which were significantly higher than normal tissues and adjacent tissues(P<0.05).The positive expression of β-catenin,Cyclin D1,MMP-9 and VEGF was significantly correlated with lymph node metastasis,depth of invasion,degree of differentiation,presence or absence of distant metastasis,Dukes stage and TNM stage(P<0.05).The age,sex,tumor location and tumor diameter of the patients were not related(P>0.05).Conclusion:The high expression of β-catenin,Cyclin D1,MMP-9 and VEGF in colon cancer is closely related to tumor metastasis and metastasis,and can be used as a molecular biological indicator to predict colon stage and lymph node metastasis.
作者
傅祥炜
徐亮
王石坚
芮选铖
曾祥勇
FU Xiang-wei;XU Liang;WANG Shi-jian;RUI Xuan-cheng;ZENG Xiang-yong(Department of General Surgery,Second Affiliated Hospital of Hainan Medical University,Haikou 570311,China)
出处
《中国现代普通外科进展》
CAS
2019年第3期188-191,共4页
Chinese Journal of Current Advances in General Surgery
