摘要
目的探讨槲皮素对大鼠宫颈炎的治疗作用及机制。方法将50只Sprague Dawley大鼠随机分为正常对照组、宫颈炎组、消糜阴道泡腾片组、低剂量槲皮素组和高剂量槲皮素组,每组10只。宫颈炎组、消糜阴道泡腾片组、低剂量槲皮素组和高剂量槲皮素组大鼠制备宫颈炎模型,将注射器插入大鼠阴道约1 cm处,注射250 g·L^(-1)苯酚胶浆0.1 mL,每日2次,连续5 d,至大鼠阴道有白色分泌物及阴道红肿、充血时,再次注射1次苯酚胶浆。对照组大鼠阴道内注射赋形剂0.1 mL。造模成功后,消糜阴道泡腾片组、低剂量槲皮素组和高剂量槲皮素组大鼠分别于阴道深处注射10 g·L^(-1)消糜阴道泡腾片溶液(50 mg·kg^(-1))、10 g·L^(-1)槲皮素溶液(25 mg·kg^(-1))、10 g·L^(-1)槲皮素溶液(100 mg·kg^(-1)),每日2次,连续10 d;对照组和宫颈炎组大鼠给予生理盐水。连续给药10 d后处死大鼠。采用苏木精-伊红(HE)染色观察大鼠宫颈组织病理形态学改变,酶联免疫吸附试验检测大鼠宫颈组织中细胞间黏附分子-1(ICAM-1)、白细胞介素-1β(IL-1β)和甘露糖结合凝集素(MBL)水平,Western blot法检测大鼠宫颈组织中核因子-κB(NF-κB) p65蛋白表达。结果 HE染色显示,正常对照组大鼠宫颈组织未见明显异常改变。宫颈炎组大鼠宫颈黏膜鳞状上皮细胞变性、坏死、脱落;黏膜及黏膜下固有层充血水肿,淋巴细胞和中性粒细胞等炎细胞浸润明显,可见部分脓性渗出物及坏死组织。与宫颈炎组比较,消糜阴道泡腾片组、低剂量槲皮素组和高剂量槲皮素组大鼠宫颈黏膜病理改变显著减轻。宫颈炎组大鼠宫颈组织中ICAM-1、IL-1β及MBL水平显著高于正常对照组(P<0.05),消糜泡腾片组、低剂量槲皮素组和高剂量槲皮素组大鼠ICAM-1、IL-1β及MBL水平显著低于宫颈炎组(P<0.05),消糜泡腾片组、低剂量槲皮素组和高剂量槲皮素组大鼠ICAM-1、IL-1β及MBL水平比较差异
Objective To investigate the mechanism of quercetin in the treatment of cervicitis in rats. Methods Fifty Sprague Dawley rats were randomly divided into normal control group,cervicitis group, Xiaomi Yindao Paotengpian group,low-dose quercetin group and high-dose quercetin group,with10 rats in each group.The rats in the cervicitis group, Xiaomi Yindao Paotengpian group,low-dose quercetin group and high-dose quercetin group were used to establish cervicitis model.The syringe was gently inserted into the vagina of rats about1 cm,and 0.1 mL phenol mortar (250 g·L^-1 ) was injected,twice a day for5 days.When the vagina of rats had white secretion,vaginal swelling and congestion,the phenol mortar was injected once again.The rats in the control group were injected with 0.1 mL excipient.After successful modeling,the rats in the Xiaomi Yindao Paotengpian group,low-dose quercetin group and high-dose quercetin group were injected with10 g·L^-1 Xiaomi Yindao Paotengpian solution (50 mg·kg^-1 ),10 g·L^-1 quercetin solution (25 mg·kg^-1 ) and10 g·L^-1 quercetin solution (100 mg ·kg^-1 ),respectively,twice a day for10 days.The rats in the control group and cervicitis group were injected with the saline.The rats were killed after10 days of continuous administration.The pathological changes of cervical tissues of rats were observed by hematoxylin-eosin (HE) staining.The levels of intercellular adhesion molecule-1 (ICAM-1),interleukin-1β( IL-1β) and mannose-binding lectin (MBL) in cervical tissues of rats were detected by enzyme linked immunosorbent assay.The expression of nuclear factor-κB (NF-κB) p65 protein in cervical tissues of rats was detected by Western blot. Results HE staining showed that there was no abnormal change in cervical tissues of rats in the normal control group.In the cervicitis group,the changes such as cervical squamous epithelial cells degeneration,necrosis, exfoliation;congestion and edema of mucosa and submucosal lamina propria,obvious infiltration of neutrophils and lymphocytes,some purulent e
作者
朱慧敏
ZHU Hui-min(Department of Obstetrics and Gynecology,the Third Affiliated Hospital of Luohe Medical College,Luohe 462000,Henan Province,China)
出处
《新乡医学院学报》
CAS
2019年第5期417-420,共4页
Journal of Xinxiang Medical University
