摘要
小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)隶属于马铃薯Y病毒科(Potyviridae)大麦黄花叶病毒属(Bymovirus),其基因组由两条正义单链RNA组成,共编码10个蛋白。先前的研究表明,马铃薯Y病毒组多种病毒编码的保守蛋白P3具有多种功能,在病毒复制、致病性、克服宿主抗性、细胞间移动等方面均具有重要作用,而P3在大麦黄花叶病毒属中是否有类似功能目前还未见报道。本研究利用烟草脆裂病毒(Tobacco rattle virus,TRV)介导的本氏烟基因沉默系统,明确了WYMV的P3碳端在本氏烟上具有致病功能域P3-C,但完整的P3则不具有致病能力。进一步移码突变研究表明,P3-C的致病性是由其编码的多肽引起的,而非病毒来源的小干扰RNA介导的宿主基因沉默。同时P3-C的两个跨膜结构域对致病性具有重要作用,单独表达任何一个跨膜结构域P3-C均不能在本氏烟上引起明显症状。
Wheat yellow mosaic virus (WYMV) belongs to the genus Bymovirus , family Potyviridae. Its genome consists of two sense single-stranded RNAs encoding a total of 10 proteins. Previous studies had shown that the conserved protein P3 encoded by potyviruses had multiple functions and played an important role in viral replication, pathogenicity, overcoming host resistance, and cell-to-cell movement. However, whether P3 had similar functions in bymovirus had never been reported yet. In this study, tobacco rattle virus(TRV)-mediated gene silencing system in Nicotiana benthamiana was used to study the function of WYMV P3. Our results indicated that C terminal of WYMV P3 (P3-C), but not the whole P3, had a pathogenic-related functional domain in N. benthamiana . Further frame shift mutation confirmed that, rather than the viral small interfering RNA-mediated host gene silencing, the pathogenicity was caused by the polypeptide of P3-C. In addition, the pathogenicity on N. benthamiana disappeared when any of the two predicted transmembrane domain in P3-C were mutated, indicating the important roles of the transmembrane domains in P3-C pathogenicity.
作者
张岩
亓玉华
鲁燕华
杨乾坤
何雨娟
李俊敏
陈剑平
ZHANG Yan;QI Yuhua;LU Yanhua;YANG Qiankun;HE Yujuan;LI Junmin;CHEN Jianping(College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002 , China;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Zhejiang Province/Ministry of Agriculture and Rural Affairs, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 , China;Institute of Plant Virology, Ningbo University, Ningbo 315211 , China)
出处
《浙江农业学报》
CSCD
北大核心
2019年第5期777-783,共7页
Acta Agriculturae Zhejiangensis
基金
国家现代农业小麦产业技术体系(CARS-3-1)
农业农村部/浙江省植保生物技术重点实验室开放课题
关键词
小麦黄花叶病毒
P3蛋白
致病性
wheat yellow mosaic virus(WYMV)
protein P3
pathogenicity
