摘要
为确定苜蓿种质材料耐盐性鉴定的适宜浓度,筛选耐盐苜蓿种质,采用常规纸上萌发试验,研究了22份苜蓿(Medicago sativa)种质材料种子相对发芽率、相对发芽势、发芽指数、胚根干质量和胚芽干质量对不同浓度(0、0.4%、0.8%、1.2%、1.6%)NaCl胁迫的响应,并对其耐盐性强弱进行聚类分析和综合评价。结果表明:随着盐胁迫增强,22份苜蓿种质材料相对发芽率、相对发芽势、发芽指数、胚根干质量和胚芽干质量较对照均显著降低;苜蓿半致死盐浓度(S_(50))均值为0.85%,变异系数26.38%,1.2%NaCl为耐盐性鉴定的适宜浓度;表现稳定的耐盐苜蓿种质材料为牧王、爱菲尼特、德国德贝和金皇后,敏盐苜蓿种质材料为敖汉、A/S综合、费纳尔和润布11。研究结果可为耐盐苜蓿新品种选育提供参考。
In order to screen salt-tolerance alfalfa germplasms and determine the appropriate concentration for salt-tolerance identification, the relative germination rate, relative germination energy, germination index, radicle dry weight and plumule dry weight were measured with conventional paper germination trial under different NaCl stress (0, 0.4%, 0.8%, 1.2%, 1.6%). Meanwhile, cluster analysis and comprehensive evaluation of salt-tolerance of 22 alfalfa germplasms were made. The results showed that relative germination rate, relative germination energy, germination index, radicle dry weight and plumule dry weight of alfalfa germplasms were all significantly lower than those of the control when the salt concentration increased. The semi-lethal salt concentration was 0.85%(variation coefficient was 26.38%), and 1.2% NaCl was the appropriate concentration to identify the salt tolerance of alfalfa. The salt-tolerant alfalfa germplasms were Herd King, Affinity, German Derby and Golden Empress, and the salt-sensitive alfalfa germplasms were Aohan, A/S Complex, Vernal and Ramble11. These results could provide references for the breeding of new salt-tolerant varieties.
作者
李明雨
王焱
梁丹妮
姚亚妮
兰剑
LI Mingyu;WANG Yan;LIANG Danni;YAO Yani;LAN Jian(School of Agriculture, Ningxia University, Yinchuan 750021 , China;College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020;Rural Practical Technology Training Center of Yuanzhou District, Guyuan 756000;Modern Grassland Engineering Technology Research Center, Ningxia University, Yinchuan 750021 , China)
出处
《浙江农业学报》
CSCD
北大核心
2019年第5期746-755,共10页
Acta Agriculturae Zhejiangensis
基金
宁夏高等学校一流学科建设(草学学科)项目(NXYLXK2017A01)
"分子育种抗逆高代材料生物学特性鉴定与牧草新种质创制"农业育种项目(2014NXYZ040203)
关键词
苜蓿
耐盐性
萌发期
聚类分析
alfalfa
salt tolerance
seed germination stage
cluster analysis
