摘要
目的探讨新型川芎嗪-香豆酸衍生物(T-CA)对拟脑缺血模型的形态保护作用。方法通过体外培养,使用神经生长因子(NGF)诱导分化PC12细胞,预先给予一定浓度(60μmol·L^(-1))的新型川芎嗪-香豆酸衍生物保护后,加入氯化钴(CoCl_2,300μmol·L^(-1))损伤细胞。采用Giemsa染色、DAPI染色、吖啶橙(AO)及吖啶橙/溴化乙锭(AO/EB)染色等方法,观察T-CA对CoCl_2损伤PC12细胞模型的形态保护作用。同时观察T-CA对大脑中动脉缺血(MCAO)模型大鼠的存活情况及脑皮层神经细胞形态的影响。结果与损伤组相比,给药组可显著抑制CoCl_2损伤PC12细胞的凋亡,缓解其形态损伤并提高MCAO模型大鼠的存活率。结论新型川芎嗪-香豆酸衍生物T-CA对CoCl_2损伤PC12细胞模型具有显著的神经保护作用,可提高MCAO模型大鼠的存活率。
Objective To study the neuroprotective effect of novel ligustrazine- coumaric acid derivative (T-CA) on the morphology of cerebral ischemia model. Methods PC12 cells were cultured and differentiated by nerve growth factor (NGF) and were pre-protected by T-CA (60 μmol ·L -1 ) before the treatment with CoCl 2 (300 μmol·L^-1 ) as an trauma model in vitro .Giemsa staining,DAPI staining,acridine orange(AO) staining and AO/EB staining were used to study the neuroprotective effect of T-CA on morphologic appearance of CoCl2-induced neurotoxic PC12 cell model.Meantime,effects of T-CA on the survival condition of middle cerebral artery occlusion(MCAO) model and morphological change of rats' cortical neuron were observed either.Results The treatment group could significantly alleviate the morphological damage of CoCl 2 -induced neurotoxic PC12 cells model and improve the survival rate of MCAO model rats. Conclusion The results showed that the novel ligustrazine derivative T-CA had a significant neuroprotective effect on CoCl 2 -induced neurotoxic PC12 cells model and could enhance the survival rate of MCAO rats at the same time.
作者
张欣钰
郭文博
王辉
吴高荣
薛南南
陈萌
房康
李国梁
王鹏龙
雷海民
ZHANG Xinyu;GUO Wenbo;WANG Hui;WU Gaorong;XUE Nannan;CHEN Meng;FANG Kang;LI Guoliang;WANG Penglong;LEI Haimin(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing100102,China)
出处
《西北药学杂志》
CAS
2019年第3期332-336,共5页
Northwest Pharmaceutical Journal
基金
国家自然科学基金项目(编号:81603256)
中华中医药学会青年人才托举工程项目(编号:CACM-2018-QNRC2-B08)
中央高校基金科研业务项目(编号:BUCM-2019-JCRC002
BUCM-2018-2020
2019-JYB-TD005)
北京中药基础与新药研究重点实验室(编号:100102)
