摘要
背景姜黄素对包括肝癌在内的多种肿瘤的发生发展表现出较好的抑制作用,但其抗肝癌的作用机制尚不完全清晰.有研究发现,姜黄素可通过调控miR-133a表达抑制胃癌细胞增殖和转移; miR-133a在肝癌中低表达的结果已有数据证实,但姜黄素是否介导miR-133a表达发挥抗肝癌的作用并不清楚.目的探讨姜黄素调节miR-133a表达对肝癌细胞迁移和侵袭的影响.方法以姜黄素(0、10、20μmol/L)处理肝癌SMMC-7721细胞48 h后, MTT法检测细胞活力, Transwell小室检测细胞的迁移和侵袭; RT-PCR检测细胞中miR-133a表达.采用RT-PCR检测正常肝LO2细胞和肝癌SMMC-7721细胞中miR-133a表达;将miR-133a模拟物转染至SMMC-7721细胞后, Transwell小室检测miR-133a对姜黄素作用前后细胞迁移和侵袭的影响.结果姜黄素能够有效抑制SMMC-7721细胞活力(10μmol/L姜黄素存活率:0.71±0.07 vs 1.02±0.09; 20μmol/L姜黄素存活率:0.45±0.05 vs 1.02±0.09)、迁移(52.32±5.48 vs121.43±12.35)和侵袭(46.33±5.38 vs109.25±10.75),上调miR-133a表达(10μmol/L姜黄素:1.62±0.11 vs 1.00±0.09; 20μmol/L姜黄素:2.96±0.25vs1.00±0.09);与LO2细胞相比,SMCC-7721细胞中miR-133a的表达水平明显降低(0.32±0.03 vs1.03±0.08);上调miR-133a表达后,SMCC-7721细胞的迁移(32.84±3.95 vs 96.35±9.08)和侵袭(42.75±5.06vs119.32±11.71)能力均明显减弱,同时姜黄素对SMCC-7721细胞迁移(29.6±3.32 vs134.62±13.41)和侵袭(31.86±4.05 vs 129.73±12.74)的抑制作用增强.结论姜黄素可通过上调miR-133a表达抑制肝癌细胞的迁移和侵袭.
BACKGROUND Curcumin has a good inhibitory effect on the occurrence and development of many kinds of tumors,including hepatocellular carcinoma,but its antihepatocellular carcinoma mechanism is not completely clear.Some studies have found that curcumin can inhibit the proliferation and metastasis of gastric cancer cells by regulating the expression of miR-133a.The low expression of miR-133a in hepatocellular carcinoma has been confirmed by data,but whether curcumin regulated expression of miR-133a plays an anti-hepatocellular carcinoma role is not clear.AIM To investigate the effect of curcumin on the migration and invasion of hepatocellular carcinoma cells and explore the underlying mechanism by detecting the expression of miR-133a.METHODS After treatment of liver cancer SMMC-7721 cells with curcumin(0,10,and 20μmol/L)for 48 h,cell viability was detected by MTT assay,cell migration and invasion were measured by transwell assay,and the expression of miR-133a in the cells was detected by RT-PCR.The expression of miR-133a in normal liver LO2 cells and hepatocellular carcinoma SMMC-7721 cells was detected by RT-PCR.After transfection with miR-133a analogue to SMMC-7721 cells,the effect of miR-133a on migration and invasion of cells before and after curcumin treatment was detected by transwell assay.RESULTS Curcumin effectively inhibited SMMC-7721 cell viability(10μmol/L curcumin:0.71+0.07 vs 1.02+0.09;20μmol/L:0.45+0.05 vs 1.02+0.09),migration(52.32±5.48 vs 121.43±12.35),and invasion(46.33±5.38 vs 109.25±10.75)and increased miR-133a expression(10μmol/L curcumin:1.62±0.11 vs 1.00±0.09;20μmol/L:2.96±0.25 vs 1.00±0.09).Compared with LO2 cells,the expression level of miR-133a(0.32±0.03 vs 1.03±0.08)in SMCC-7721 cells was decreased obviously.After increasing the expression of miR-133a,the migration(32.84±3.95 vs 96.35±9.08)and invasion(42.75±5.06 vs 119.32±11.71)of SMCC-7721 cells were significantly decreased,and the inhibitory effect of curcumin on migration(29.6±3.32 vs 134.62±13.41)and invasion(31.8
作者
袁洪波
孟佩盈
戚柳杰
Hong-Bo Yuan;Pei-Ying Meng;Liu-Jie Qi(Second Department of Internal Medicine,Zhuji Central Hospital,Zhuji 311800,Zhejiang Province,China)
出处
《世界华人消化杂志》
CAS
2019年第8期477-484,共8页
World Chinese Journal of Digestology
