摘要
为掌握湖北省花生种植区土壤中黄曲霉菌的分布和产毒特征,从罗田、红安、钟祥、襄阳四个典型花生种植区采集土壤样品40份,并进行黄曲霉菌分离、鉴定和产毒力研究。研究结果表明:湖北省不同花生种植区共分离鉴定到黄曲霉菌51株,土壤中黄曲霉菌落数为127.5cfu/g。不同种植区土壤中黄曲霉菌分布存在显著差异,钟祥土壤中黄曲霉菌落数最高,罗田最低;鉴定获得黄曲霉菌株中产毒菌株占96%,产毒量范围0～227.81μg/L,不产毒菌株占4%;产毒菌株分为只产AFB_1、产AFB_1+AFB_2、产AFB_1+AFB_2+AFG_1和产AFB_1+AFB_2+AFG_1+AFG_2毒素4种类型,其中以产AFB_1+AFB_2的菌株占比最高,为65%;不同种植区黄曲霉菌株产毒力研究发现,钟祥每克土壤中黄曲霉菌产AFB_1的量最高,达11 679.70μg/L。本研究可为湖北花生黄曲霉毒素污染预警和防控提供理论依据。
To study the occurence and distribution of aflatoxin by Aspergillus flavus in soils,samples from the typical peanut areas in Hubei Province were collected.In total 40 soil samples from Luotian,Hongan,Zhongxiang and Xiangyang were collected for A.flavus isolation,identification and aflatoxin detection.Results showed that 51 strains were isolated from the 4 areas.The population density of A.flavus was 127.5 cfu/g in one gram soil.But the population of A.flavus in soils of different peanut areas were significantly different.The highest populations of A.flavus colonies were found in Zhongxiang,and those from Luotian were the lowest.Among the strains,aflatoxigenic strains accounted for 96%,with AFT content of 0-227.81μg/L,and atoxigenic strains was 4%.There were 4 types of aflatoxigenic strains,which were strains only producing AFB1,strains producing AFB1+AFB2,strains producing AFB1+AFB2+AFG1 and strains producing AFB1+AFB2+AFG1+AFG2.Among them,strains producing AFB1+AFB2 accounted for the highest proportion,reaching 65%.Studies on aflatoxin production in different planting areas found that the highest amount of AFB1 contamination per gram of soil was in Zhongxiang,reaching 11 679.70μg/L.This study provided a theoretical base for predicting and controlling of aflatoxin contamination in typical peanut planting areas in Hubei Province.
作者
朱婷婷
陈琳
岳晓凤
白艺珍
丁小霞
李培武
张奇
张文
ZHU Ting-ting;CHEN Lin;YUE Xiao-feng;BAI Yi-zhen;DING Xiao-xia;LI Pei-wu;ZHANG Qi;ZHANG Wen(Oil Crops Research Institute of the Chinese Academy of Agriculture,Wuhan 430062,China;Laboratory of Risk Assessment for oilseeds Produets(Wuhan),Ministey of Agriculture and Rural Affairs,Wuhan 430062,China;Key Laboratory of Detection for Mycotoxins,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;National Agricultural Testing Benchmark Laboratory ( Biotoxin ),Wulum 430062,China;China Quality Inspection and Test Center for Oilseeds Products,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2019年第2期255-260,共6页
Chinese Journal of Oil Crop Sciences
基金
"十三五"国家重点研发计划(2017YFC1601201)
国家自然科学基金(31601573
31701726)
关键词
花生
土壤
黄曲霉菌
湖北省
分布
产毒力
peanut
soil
Aspergillus flavus
Hubei Province
distribution
aflatoxin production
