摘要
【目的】比较猪舍类型对舍内气溶胶致病菌数量和内毒素水平的影响。【方法】选用32栋全封闭式猪舍,共计4种类型(保育舍、育肥舍、妊娠舍和分娩舍),每种类型猪舍8栋。采用AGI-30采样器和安德森6级采样器收集空气样品,用平板培养和RT-qPCR的方法测定需氧菌和致病菌的丰度,并测定气溶胶中内毒素的含量。【结果】保育舍需氧菌数量显著高于其他猪舍,而分娩舍需氧菌数量显著低于其他猪舍(P<0.05)。保育舍气溶胶中弯曲杆菌和大肠埃希菌的数量显著高于其他猪舍(P<0.05)。此外,保育舍气溶胶中内毒素含量显著高于其他猪舍(P<0.05)。【结论】不同猪舍类型气溶胶中致病菌数量和内毒素含量存在差异。
【Objective】The present study was designed to compare the abundances of pathogens and en-dotoxin between different piggeries types.【Method】In summer,a total of 32 swine buildings,which were sorted into 4 categories including nursery piggery,finishing piggery,gestation piggery and farrowing piggery (n=8),were chosen to collect aerosol samples using AGI-30 impingers and six-stage Andersen microbial samplers. The abundances of aerobic bacteria and pathogens were determined using culturedependent method or RT-qPCR. In addition,the concentration of endotoxin in aerosol samples was also monitored.【Result】The colonies number of aerobic bacteria was higher in aerosols of nursery building than that in aerosols of other swine buildings (P<0.05),and the total number of aerobic bacteria was lower in aerosols of farrowing building than that in aerosols of other swine buildings (P<0.05). The abundances of aerosol Campylobacter and E. coli in nursery piggery was higher compared with other piggeries types (P<0.05). Additionally,the nursery barns exhibited higher concentration of endotoxin than other barns (P< 0.05).【Conclusion】It can be concluded that piggery categories significantly influence the abundances of aerosol pathogens and endotoxin.
作者
许铭洙
张勇
郭振东
严鸿林
张宏福
刘静波
XU Mingzhu;ZHANG Yong;GUO Zhendong;YAN Honglin;ZHANG Hongfu;LIU Jingbo(School of Life Science and Engineering,Southwest University of Science and Technology, Mianyang 621010,Sichuan,China;Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122,China;State Key Laboratory of Animal Nutrition,Institute of Animal Sciences, Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《四川农业大学学报》
CSCD
北大核心
2019年第2期271-275,共5页
Journal of Sichuan Agricultural University
基金
国家重点研发计划项目(2016YFD0500505)
