摘要
淋巴细胞脉络丛脑膜炎病毒(lymphocytic chorimeningtis virus,LCMV)是一种经鼠传播的人畜共患病毒。为明确东北地区LCMV在蜱的感染本底,2015年在吉林省采集蜱534只,其中草原革蜱占43.1%;森林革蜱38.4%;长角血蜱11.4%和全沟硬蜱7.1%。将采集蜱按蜱种及地区分组,进行宏病毒组测序,发现蜱感染LCMV。通过RT-PCR检测发现吉林省LCMV蜱感染率为3.5%,其中长角血蜱、草原革蜱、森林革蜱和全沟硬蜱感染率分别为12.2%,2.5%,4.4%和2.5%,长角血蜱携带率最高。通过全基因组测序获得LCMV全基因组序列,并对其进行遗传进化分析,结果表明东北地区蜱感染的LCMV属于基因Ⅰ型,提示蜱可能作为LCMV的传播媒介。本研究确定了东北地区蜱的LCMV感染率及基因型,为LCMV的防控监测提供科学依据。
Lymphocytic chorimeningtis virus(LCMV) is an important rodent-borne zoonotic pathogen,and ticks may be as vector for the virus.In this study,ticks were collected in Dunhua City and Jingxin Town in Jilin Province in 2015,and were performed metagenomic sequencing.This study collected 534 ticks of four species: D.nuttalli(43.1%) D.silvarum(38.4%),H.longicornis(11.4%),I.persulcatus(7.1%).Ticks carrying rate of LCMV was 3.5% in Jilin Province.Prevalecne of H.longicornis,D.nuttalli,D.silvarum,and I.persulcatus carrier rates of LCMV were 12.2%,2.5%,4.4%,and 2.5%,respectively.This experiment successfully obtained the complete genome sequence of LCMV carried by four species and analyzed genetic evolution.Full-length genome of thes viruses were grouped with the lineage I strains that are associated with severe diseases in humans.This research determined the rate of ticks carrying the LCMV in northeast China.We provide a scientific basis for prevention and control and monitoring of genetic variation of ticks carrying LCMV.
作者
李爽
王泽东
张力
王立富
魏峰
II Shuang;WANG Ze-dong;ZHANG Li;WANG Li-fu;WEI Feng(College of Life Science Jilin Agricultural University ,Changchun 130118 China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2019年第3期451-455,共5页
Chinese Journal of Veterinary Science
基金
国家重点研发计划基金资助项目(2017YF-D0501702)
