摘要
目的观察皮肤桥蛋白(Dermatopontin, DPT)对食道癌细胞增殖能力的影响。方法应用基因沉默技术在食道癌细胞Eca-109中下调DPT基因的表达,通过Western blot实验与免疫荧光实验评价24h、48h、72h基因沉默效率;应用克隆形成实验及MTT实验,检测下调DPT基因对食道癌细胞增殖能力的影响;流式细胞术检测细胞周期;通过Western blot实验检测CyclinD1蛋白的表达变化。结果抑制DPT基因可以下调人食道癌细胞的克隆形成与细胞增殖能力,沉默DPT基因引起食道癌细胞G 0/G1期阻滞, CyclinD1蛋白表达下调。结论 DPT参与食道癌的发病机制,是食道癌治疗的新靶点。
Objective To investigate the effect of Dermatopontin(DPT) gene on the proliferation of esophageal squamous carcinoma cells(ESCCs). Methods DPT in Eca-109 cell lines was silenced, and the transfection rate was evaluated by immunofluorescence staining and Western blot assay. The proliferation of Eca-109 cells was measured by MTT method and colony formation assay. The flow cytometry and Annexin Vfluorescein isothiocyanate assay were used to detect cell cycle, and CyclinD1 expression was detected by Western blot. Results DPT gene silencing was able to significantly reduce the proliferation of Eca-109 cells, the proportion of cells in the G /G phase increased significantly, while the 0 1 expression level of CyclinD1 was up-regulated. Conclusion DPT gene is involved in the pathogenesis of ESCC, might be a novel target for the treatment of ESCC.
作者
甘颖
李虹怡
GAN Ying;LI Hong-yi(Department of Gastroenterology, Huludao City Central Hospital in Longwan District, Hu ludao125000, China)
出处
《解剖科学进展》
2019年第2期136-138,共3页
Progress of Anatomical Sciences
