摘要
目的本研究旨在探讨miR-874-3p调控胶质瘤血管生成拟态的潜在分子机制。方法应用实时定量PCR检测miR-874-3p在胶质瘤细胞系U87细胞中的表达水平;应用双荧光素酶报告基因分析系统检测miR-874-3p和SDC1的结合作用;应用CCK-8法、transwell法、体外管形成实验检测miR-874-3p或SDC1表达变化对U87细胞增殖、迁移、侵袭和管形成能力的影响。结果 miR-874-3p在U87细胞中低表达,过表达miR-874-3p显著抑制U87细胞的增殖、迁移、侵袭和管形成能力。SDC1在U87细胞中的表达上调,沉默SDC1显著抑制U87细胞增殖、迁移、侵袭和管形成能力。miR-874-3p靶向SDC1 3'UTR。结论 miR-874-3p通过靶向结合SDC1抑制胶质瘤血管生成拟态。
Objective To investigate the potential molecular mechanisms by which miR-874-3p regulated vasculogenic mimicry formation in U87 cells. Methods Quantitative real-time PCR was used to analyze the expression of miR-874-3p in U87 cells, luciferase reporter assay was performed to confirm the binding site between miR-874-3p and SDC1. CCK-8, transwell, in vitro VM tube formation assay were used to evaluate the effects of miR-874-3p or SDC1 on cell proliferation, migration, invasion and the formation ability of VM tube. Results MiR-874-3p was downregulated in U87 cell compared with primary normal human astrocytes, whereas overexpression of miR-874-3p inhibited proliferation, migration, invasion, as well as the formation ability of VM tube in U87 cell. SDC1 was upregulated in U87 cells compared with NHA, and knockdown of SDC1 inhibited VM formation of U87 cells. MiR-874-3p directly targeted the 3'UTR of SDC1. Conclusion MiR-874-3p inhibited glioma vasculogenic mimicry formation via targeting SDC1.
作者
董一明
刘丽波
薛一雪
刘云会
DONG Yi-ming;LIU Li-bo;XUE Yi-xue;LIU Yun-hui(Department of Neurosurgery, Shengjing Hospital,China Medical University, Shenyang 110004, China;Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang 110004, China)
出处
《解剖科学进展》
2019年第2期123-126,共4页
Progress of Anatomical Sciences
基金
国家自然科学基金(81672511)
盛京自由研究者计划(201304)
