摘要
目的:探讨KRT18对软骨肉瘤细胞增殖、迁移、侵袭和凋亡的影响。方法:采用脂质体法将pcDNA3.1,pcDNA3.1-KRT18,siControl和siKRT18转染到软骨肉瘤细胞SW1353;采用qRT-PCR法检测细胞中KRT18的表达;Western印迹法检测细胞中KRT18蛋白表达;MTT法检测细胞的增殖率;Transwell检测细胞的迁移和侵袭;流式细胞术检测细胞的凋亡率。结果:与软骨细胞HC-A相比,软骨肉瘤细胞SW1353中KRT18的表达显著上调,且过表达KRT18可显著上调SW1353细胞增殖、迁移、侵袭,显著下调细胞凋亡(P<0.05);沉默KRT18可抑制细胞增殖、迁移、侵袭并促进凋亡。结论:沉默KRT18可抑制软骨肉瘤细胞增殖、迁移、侵袭,促进凋亡,将可为软骨肉瘤的治疗提供新方向。
Objective: To investigate the effects of KRT18 on cell proliferation, migration, invasion and apoptosis of chondrosarcoma cells. Methods: pcDNA3.1, pcDNA3.1-KRT18, siControl and siKRT18 were transfected into chondrosarcoma cell SW1353 by liposome method. The expression of KRT18 was detected by qRT-PCR. The protein of KRT18 was detected by Western blot. Cell proliferation rate was detected by MTT assay;cell migration and invasion were detected by Transwell;flow cytometry was used to detect cell apoptosis rate. Results: Compared with chondrocyte HC-A, the expression of KRT18 in chondrosarcoma cell SW1353 was significantly up-regulated, and overexpression of KRT18 significantly up-regulated the proliferation, migration and invasion of SW1353 cells, and significantly down-regulated apoptosis (P<0.05). Silencing KRT18 inhibited cells proliferate, migrate, invade and promote apoptosis. Conclusion: Silencing KRT18 could inhibit cell proliferation, migration, invasion and promote apoptosis of chondrosarcoma cells, which will provide a new direction for the treatment of chondrosarcoma.
作者
王硕
孙飞
徐海伟
WANG Shuo;SUN Fei;XU Haiwei(Department of Orthopaedics, Zaozhuang Hospital, Zaozhuang Mining Group, Zaozhuang Shandong 277100, China)
出处
《临床与病理杂志》
2019年第4期698-705,共8页
Journal of Clinical and Pathological Research
关键词
KRT18
软骨肉瘤细胞
增殖
迁移
侵袭
凋亡
KRT18
chondrosarcoma cells
proliferation
migration
invasion
apoptosis
