摘要
以野菊为材料,研究了乙醇浓度、提取时间、回流次数对多糖提取率的影响,并在单因素试验的基础上,以滤渣中酚酸的含量及提取液中多糖的含量为指标,进行了3因素3水平的正交试验。结果表明:最佳工艺为4倍量95%的乙醇回流提取2次各1 h,该条件野菊多糖获得量最优,酚酸杂质含量最低。该工艺稳定可行,可为野菊多糖大规模提取作依据。
The effect of ethanol concentration, extraction time and reflux times on the extraction rate of polysaccharide was studied by using Chrysanthemumindicum L.. Based on the single factor experiment, the content of phenolic acid in the residue and the content of polysaccharide in the extract were used as indicators for a threefactor and three-level orthogonal experiment. The results showed that the optimal process was 4 times and 95% ethanol refluxed for 1 h two times. Under this condition the yield of polysaccharide from Chrysanthemum indicum L.was highest, and the phenolic acid impurity content was the lowest. The process is stable and feasible, and can be used as a basis for large-scale extraction of wild chrysanthemum polysaccharide.
作者
高俊明
刘伟
李斌
陈磊
田薇
GAO Junming;LIU Wei;LI Bin;CHEN Lei;TIAN Wei(College of Forestry and Biotechnology,Zhejiang A & F University,Lin’an 311300,Zhejiang;Lishui baiyun mountain forest farm, Lishui 323000, Zhejiang)
出处
《丽水学院学报》
2019年第2期56-60,共5页
Journal of Lishui University
基金
浙江农林大学学生科研创新项目"植物源诱导子CIP对栽培白术药效成分的影响"(102-2013200014)
关键词
野菊多糖
提取工艺
正交实验
酚酸含量测定
多糖含量测定
polysaccharide
extraction technology
orthogonal experiment
content
content of polysaccharide
