摘要
【目的】为了筛选出能高效降解渗滤液中氨氮或TN的菌株。【方法】在高浓度的初始氨氮浓度条件下从北京市南宫堆肥厂垃圾渗滤液中筛选出一株能高效降解氨氮的菌株SDB1-2,通过形态观察、革兰氏染色和16S-rRNA分子鉴定,鉴定该菌株为肺炎克雷伯氏菌Citrobacter freundii,简称SDB1-2。【结果】经研究发现,其能降解初始氨氮浓度高达2 000mg/L的人造废水。在不添加任何其他物质的条件下,该菌株对北京市某综合垃圾处理厂渗滤液中氨氮,TN和COD均能实现一定程度的降解,对同一批垃圾渗滤液进行高温高压处理后,同等条件下其最高去除率分别为51.15%、35.03%、57.45%。【结论】为环保工作者开发垃圾渗滤液处理生化工艺中氨氮、TN或COD的高效去除提供理论依据和技术支持。
The main pollutants of landfill leachate are ammonia nitrogen,TN,COD,etc.Screening strains capable of efficiently degrading ammonia nitrogen or TN is of great significance for the treatment of landfill leachate.In this study,a strain of SDB1-2 capable of efficiently degrading ammonia nitrogen was screened from the landfill leachate of Beijing Nangong Composting Plant under high concentration of initial ammonia nitrogen concentration,and it was identified by morphological observation,Gram stain and 16S-rRNA molecular identification.The strain was identified as Citrobacter freundii,referred to as SDB1-2.It has been found that it can degrade artificial wastewater with an initial ammonia nitrogen concentration of up to 2000 mg/L.Under the condition of not adding any other substances,the strain can achieve a certain degree of degradation of ammonia nitrogen,TN and COD in the leachate of a comprehensive waste treatment plant in Beijing.After the same batch of landfill leachate is treated under high temperature and high pressure,the same conditions The highest removal rates were 51.15%,35.03%,and 57.45%,respectively.This study aims to provide theoretical basis and technical support for environmental protection workers to develop high-efficiency removal of ammonia nitrogen,TN or COD in the biochemical process of landfill leachate treatment.
作者
张超
尚润东
刘宇璇
杨国庆
李璐
施磊
靳永胜
ZHANG Chao;SHANG Rundong;LIU Yunxuan;YANG Guoqing;LI Lu;SHI Lei;JIN Yongsheng(Beijing Institute of Urban Management,Beijing 102206,China;College of Biological Science and Engineering,Beijing Agricultural University,Beijing 102206,China)
出处
《北京农学院学报》
2019年第2期1-4,共4页
Journal of Beijing University of Agriculture
