摘要
本文以壳聚糖作为壁材,鲎血细胞蛋白为芯材,以微胶囊粒径、表观为判断标准,通过正交实验,采用化学交联法制备微胶囊,并测试其释放率。结果确定鲎血细胞蛋白微胶囊的最佳制备工艺:2%壳聚糖在45℃水浴中按500 r/min转速搅拌,加入3%的25%戊二醛制备出粒径小于200μm微胶囊,且表观圆整光滑,包封率达到85.95%。微胶囊在模拟胃液和模拟肠液中五小时后的蛋白释放率分别是29.19%和36.48%,在模拟肠液中的蛋白释放率高于模拟胃液,减少了鲎血细胞蛋白在胃液里的损耗。
Based on chitosan as wall material, and blood protein as core material, regarding microcapsule particle size and apparent as the judgment standard, microcapsules were prepared by chemical crosslinking through the orthogonal experiment, and its releasing rate were also tested. Result has shown the best preparation technology of blood-cell protein microcapsule: 2% chitosan was stirred in 45 ℃ water bath at a speed of 500 rpm, and then adding 3% of 25% glutaraldehyde solution, the obtained microcapsules were apparently round and smooth with a particle size of less than 200 microns, their encapsulation rate could reach 85.95%. The protein release rate of microcapsule in simulated gastric juice and simulated intestinal juice for five hours were 29.19% and 36.48% respectively, which the protein release rate in simulating intestinal juice was higher than that in simulated gastric juice, helpful for reducing loss of the blood-cel protein in gastric juice.
作者
梁惠聪
黄丽玲
邓春梅
何兰珍
张国光
吴育廉
洪鹏志
康信煌
Liang Huicong;Huang Liling;Deng Chunmei;He Lanzhen;Zhang Guoguang;Wu Yulian;Hong Pengzhi;Kang Xinhuang(College of Chemistry and Environment Guangdong Ocean University, Zhanjiang 524088;College of Food Science and Technology Guangdong Ocean University, Zhanjiang 524088, China)
出处
《广东化工》
CAS
2019年第8期32-35,63,共5页
Guangdong Chemical Industry
基金
广东海洋大学自然科学基金(Q17092
Q15094
E13503
Q14176)
湛江市科技局自然科学基金(A15445
A14457
2016A02023)
湛江市海洋经济发展创新发展示范市建设项目(湛海创2017C8B1)
国家级大学生创新创业训练计划项目(CXXL2018033)
关键词
鲎
血细胞
蛋白
壳聚糖
微胶囊
Tachypleus tridentatus
blood-cell
protein
chitosan
microencapsulatio
