摘要
旨在比较研究转化生长因子-β1 (transforming growth factor-β1, TGF-β1)及其受体在性成熟前后藏绵羊睾丸组织的表达及分布特征,探索其对藏绵羊睾丸发育及生殖机能的调节作用。采用睾丸摘除手术收集1.0、1.5、2.0岁藏绵羊睾丸,应用免疫印迹、免疫组织化学SP法及IPP图像分析研究TGF-β1及其受体(transforming growth factor-βreceptor I, TGF-βR I)的表达及分布特征。结果表明:1)TGF-β1在藏绵羊睾丸组织中弱表达,性成熟前后差异不显著(P>0.05);1.5岁藏绵羊睾丸组织中TGF-βRⅠ表达均显著高于1.0及2.0岁(P<0.05)。2)各年龄组TGF-β1主要表达于睾丸间质细胞(Leydig)及小血管内皮细胞,1.5岁藏绵羊在各级生精细胞及支持细胞(Sertoli)亦有中等阳性表达,其他年龄组在生精上皮弱表达;TGF-βRⅠ在各年龄组生精上皮均有表达,1.0岁时在Leydig细胞无明显表达,1.5岁时强表达于圆形精子、Leydig细胞及淋巴管内皮细胞,2.0岁时在Leydig细胞及毛细淋巴管亦见阳性表达,但明显弱于1.5岁。3)各年龄组TGF-βRⅠ表达明显强于TGF-β1,TGF-β1表达组间差异不显著(P>0.05);TGF-βRⅠ在1.5岁时较1.0及2.0岁表达差异显著(P<0.05)。综上表明,高原地区藏绵羊睾丸组织TGF-β1和TGF-βRⅠ的阳性表达随着性成熟出现先上升后下降的变化趋势,提示性成熟前后TGF-β1及其受体通过调节Sertoli细胞在精子生成过程中发挥作用;各年龄段藏绵羊睾丸组织TGF-βRⅠ及TGF-β1阳性表达以Leydig细胞变化最为明显,为进一步分析TGF-β1/TGF-βRⅠ信号通路随着性成熟发育参与调节Leydig细胞生物合成提供研究基础。
This experiment was conducted to compare the distribution characteristics of TGF-β1 and its receptors(TGF-βRⅠ)in Tibetan sheep testis before and after sexual mature and attempt to explore their relationships with testicular spermatogenesis in sexual mature.The testis of 1.0-,1.5-,2.0-year-old Tibetan sheep were prepared by testicular removal surgery for describe the distribution density and the location of TGF-β1 and TGF-βRⅠin the testis tissues using the Western blot,immunohistochemical SP combined with IPP(Image-Pro Plus)statistics methods.The results suggested that:1)The reactions of TGF-β1 antibody were weakly and there were no significant difference in Tibetan sheep testis before and after sexual mature(P>0.05);The expression of TGF-βRⅠin the 1.5-year-old sheep testis tissue were significantly higher than 1.0-and 2.0-year-old(P<0.05).2)The TGF-β1 was mainly present in Leydig cells and endothelial cells of small vascular at different ages,there were medium positive expression of TGF-β1 in Sertoli cells and spermatocytes at 1.5-year-old Tibetan sheep but weakly expressed in seminiferous epithelium at other 2 groups.The TGF-βRⅠwere expressed in seminiferous epithelium at 3 ages.In the 1.0-year-old sheep testis,the expression of TGF-βRⅠwas weakly expressed in Leydig cells,and at the age of 1.5,its expression in the round spermatid,Leydig cells and lymphatic endothelial cells were strongly positive,also there were immunolocalized in Leydig cells and capillary lymph vessel at 2-year-old,basically the expression of TGF-βRⅠin the 1.5-year-old sheep testis was more stronger than that of other 2 groups.3)The positive signal statistics indicated that the average absorbance of TGF-βRⅠexpression was significantly higher in the testis than TGF-β1,and the expression of TGF-β1 were not different in the testis of the Tibetan sheep before and after sexual mature(P>0.05),the TGF-βRⅠwas expressed significantly higher in testis tissue of 1.5-year-old Tibetan sheep than that of 1.0-and 2.0-year-old
作者
袁莉刚
宋笑
杨婕
梁霞霞
陈国娟
YUAN Ligang;SONG Xiao;YANG Jie;LIANG Xiaxia;CHEN Guojuan(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第4期750-757,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
甘肃农业大学科技创新基金-青年导师基金项目(GAU-QDNS-201604)
国家自然科学基金(31160488)
