摘要
目的观察蒙医不同针刺法对抑郁大鼠前额叶神经递质及蛋白激酶(PKA)活性、环磷腺苷效应元件结合蛋白(CREB)磷酸化和脑源性神经营养因子(BDNF)表达,探究蒙医不同针法的抗抑郁作用机制。方法将40只雄性Sprague-Dawley大鼠按照随机数字表法分为空白组、模型组、蒙医银针组、蒙医三根平衡针组和药物组,每组8只。除空白组外其他组大鼠孤养并给予不可预知的7种不同的应激刺激28 d。其中空白组每笼饲养4只,正常饮水摄食,不给任何刺激;模型组不给予任何治疗;蒙医银针组于每日接受应激刺激前1 h,进行蒙医银针刺激巴达干穴、心穴和黑白际穴;蒙医三根平衡针组的针刺和选穴同蒙医银针组,但每日选取1个穴位给予温针刺激;药物组于每日进行应激刺激前1 h,将盐酸氟西汀以0.9%氯化钠溶液配制成1 mg/mL,按照2 mg/kg体重,灌胃给药。28 d后断头,取大鼠前额叶,采用酶联免疫吸附法和Western blot法检测5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)含量,PKA活性,CREB磷酸化和BDNF表达水平。结果与空白组比较,造模28 d后模型组大鼠前额叶5-HT、NE、DA含量显著降低(P <0.01),PKA活性、CREB和BDNF表达水平也明显下降(P <0.05)。与模型组比较,蒙医三根平衡针组、药物组大鼠前额叶NE、DA和5-HT的含量明显升高,差异有统计学意义(P <0.05),蒙医银针组大鼠前额叶DA和5-HT的含量明显升高,差异有统计学意义(P <0.05)。与模型组比较,蒙医银针组、蒙医三根平衡针组和药物组大鼠前额PKA活性、CREB和BDNF表达水平显著升高,差异有统计学意义(P <0.05)。结论蒙医不同针法能有效提高5-HT、NE、DA含量、PKA活性及CREB-BDNF的表达,提示蒙医不同针法均有抗抑郁作用。
Objective To observe the effects of different acupuncture methods on the neurotransmitter and protein kinase (PKA) activity, cyclophosphamide receptor binding protein (CREB) phosphorylation and brain-derived neurotrophic factor (BDNF) expression in the prefrontal cortex of depressed rats. To explore the antidepressant mechanism of different acupuncture methods in Mongolian medicine. Methods A total of 40 male Sprague-Dawley rats were divided into blank group, model group, Mongolian medical silver needle group, Mongolian medicine three balanced needle group and drug group according to random number table method, with 8 rats in each group. Rats in the other groups were orphaned except for the blank group and were given unpredictable 7 different stress stimulation for 28 days. Among them, 4 rats in the blank group were raised in each cage and fed with normal drinking water without any stimulation;the model group did not receive any treatment;the Mongolian medical silver needle group received the silver needle for stimulation of the Bada dry point, Heart acupuncture points and black and white points 1 h before the daily stress stimulation;acupuncture and acupuncture points of the Mongolian medicine three balanced needle group were the same as those in the Mongolian medical silver needle group, but one acupoint was selected daily for warm needle stimulation;the drug group was given 1 hour before stress stimulation daily, Fluoxetine hydrochloride was formulated into a 1 mg/1 mL solution in a 0.9% sodium chloride solution, and the drug group received intragastric administration according to a body weight of 2 mg/kg. After 28 days, the rats were decapitated and the prefrontal lobe was taken. The 5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA) content, PKA activity, CREB phosphorylation and BDNF expression level were detected by enzyme-linked immunosorbent assay and Western blot. Results Compared with the blank group, the contents of 5-HT, NE and DA in the prefrontal cortex of the model group were significa
作者
艾丽雅
杨利娟
刘俊彤
敖其尔
赛音朝克图
AI Liya;YANG Lijuan;LIU Juntong;AO Qier;Saiyinchaoketu(Graduate School, Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010110, China;School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;the Fifth Department of Rehabilitation, Inner Mongolia International Mongolian Hospital, Inner Mongolia Autonomous Region, Hohhot 010065, China)
出处
《中国医药导报》
CAS
2019年第8期9-12,38,共5页
China Medical Herald
基金
国家自然科学基金资助项目(81760909)
