摘要
目的通过模拟临床高蛋白高钙饮食诱导迪法克鹌鹑痛风,初步探讨建立鹌鹑痛风模型的方法,为痛风发病机制研究及防治药物筛选提供依据。方法选用36只30日龄雄性健康迪法克鹌鹑,以体重和血尿酸水平随机分为3组,即对照组、模型I组和模型II组,每组12只。对照组鹌鹑饲喂基础日粮,模型组饲喂高蛋白高钙日粮(日粮粗蛋白含量29.5%,钙含量7.8%),对照组、模型I组自由饮水,模型II组每日限制饮水4 h,实验持续50 d。检测鹌鹑血清、粪尿尿酸水平,同时检测肾功能并进行组织学病理切片观察。结果高蛋白高钙日粮可使鹌鹑血清尿酸水平显著升高;粪尿尿酸水平显著降低;血清肌酐、尿素氮水平升高;导致鹌鹑肾尿酸盐沉积明显。高蛋白高钙日粮合并限制饮水可加剧血尿酸水平升高、肾尿酸盐沉积,使部分鹌鹑出现关节肿大、变形等痛风症状。结论饲喂高蛋白高钙日粮并限制饮水量可以建立痛风鹌鹑模型,其发病过程与临床痛风病因、发病机制及临床表现更为符合,为痛风病的基础及药物开发研究提供参考。
Objective To establish a quail model of gout by simulating a clinical high-protein and high-calcium diet,and to provide a basis for studying gout pathogenesis and drug screening for its prevention and treatment.Methods Tirty-six 30-day-old healthy male quails were randomly and equally divided into three groups according to body weight and blood uric acid level,namely,the control group,model I group,and model II group.The control group was fed the basal diet,and the model groups were fed a high-protein high-calcium diet (dietary crude protein content of 29.5% and calcium content of 7.8%).The control group and the model I group were provided with drinking water ad libitum,while the model II group was given drinking water restricted to 4 h daily.The experiment lasted for 50 days.The serum and fecal uric acid levels were measured,renal function was determined,and histopathological examination was performed.Results The high-protein and high-calcium diet significantly increased serum uric acid levels,significantly reduced fecal uric acid levels,and did not change the serum creatinine and urea nitrogen levels.The high-protein and high-calcium diet combined with restricted drinking water aggravated the increase of blood uric acid level and renal urate deposition,causing gout changes such as joint swelling and deformation.Conclusions A gout model can be established by feeding on a high-protein and high-calcium diet and limiting the amount of drinking water.The pathogenesis of gout in this model is more consistent with the etiology,pathogenesis,and clinical manifestations of gout in clinics,providing a reference for the research on gout pathogenesis and drug development.
作者
王海鸽
林志健
张冰
李高玺
王笑
WANG Haige;LIN Zhijian;ZHANG Bing;LI Gaoxi;WANG Xiao(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第4期15-22,共8页
Chinese Journal of Comparative Medicine
基金
国家重大新药创制(2017ZX09301024)
国家自然科学基金(81673618)
北京市自然科学基金(7162117)
关键词
鹌鹑
痛风
高蛋白高钙饮食
尿酸盐沉积
高尿酸血症
quail
gout
high-protein and high-calcium diet
urate deposition
hyperuricemia
