摘要
目的分析肝细胞癌(HCC)组织中活化T细胞核因子(NFAT)各亚型的表达情况及其与临床病理因素的关系。方法回顾分析2014年9月至2016年12月天津市第一中心医院肝胆外科行肝切除术的105例HCC患者资料,男性87例,女性18例,年龄(55.1±10.8)岁。采用免疫组化染色检测HCC组织和癌旁正常肝组织中NFAT各亚型的表达情况,分析NFAT各亚型表达的差异及相关因素。结果HCC组织中NFAT1阳性表达比例低于癌旁组织,而NFAT4阳性比例高于癌旁组织,差异有统计学意义(P<0.05)。NFAT1表达阳性组的HBV比例高于阴性组(93.1%比78.7%),微血管侵犯比例低于阴性组(24.1%比46.8%),差异有统计学意义(P<0.05)。NFAT3表达阳性组≤60岁比例高于阴性组(80.0%比60.0%),微血管侵犯比例高于阴性组(46.2%比15.0%),差异有统计学意义(P<0.05)。NFAT4表达阳性组微血管侵犯比例高于阴性组(43.3%比22.2%),差异有统计学意义(P<0.05)。结论NFAT各亚型在HCC组织中存在差异化表达,NFAT1、NFAT3与NFAT4的表达与微血管侵犯相关。
Objective To analyze the expression of activated T cell nuclear factor (NFAT) in hepatocellular carcinoma (HCC) tissues and its correlation with clinicopathological factors. Methods Data of 105 patients including 87 males and 18 females, aged 55.1±10.8 years old, diagnosed with HCC who underwent hepatectomy in hepatobiliary surgery department of the first central hospital of Tianjin from September 2014 to December 2016 were retrospectively analyzed. Immunohistochemical staining was used to detect the expression of NFAT subtypes in HCC tissues and adjacent normal liver tissues, and the differences in expression of NFAT subtypes and related factors were analyzed. Results HCC tissues had higher expression of NFAT4 and lower expression of NFAT1 compared to adjacent tissues (P<0.05). NFAT1 positive group had higher HBV infected rate (93.1% vs. 78.7%) and lower microvascular invasion rate than that in NFAT1 negative group (24.1% vs.46.8%)(P<0.05). NFAT3 positive group had more younger patients (≤60 years old)(80.0% vs. 60.0%) and higher microvascular invasion rate (46.2% vs. 15.0%)(P<0.05). NFAT4 positive group had higher microvascular invasion rate (43.3% vs. 22.2%)(P<0.05). Conclusion HCC tissues had different expressions of NFATs. The expressions of NFAT1, NFAT3 and NFAT4 are related to microvascular invasion.
作者
柴嘉穗
王建
刘子荣
李妍
李志胜
牛连杰
张雅敏
Chai Jiasui;Wang Jian;Liu Zirong;Li Yan;Li Zhisheng;Niu Lianjie;Zhang Yamin(The First Central Clinical College of Tianjin Medical University, Tianjin 300192, China;Department of Hepatobiliary Surgery, Tianjin First Center Hospital, Tianjin 300192, China;Biobank of Tianjin First Center Hospital, Tianjin 300192, China)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2019年第3期171-174,共4页
Chinese Journal of Hepatobiliary Surgery
基金
天津市卫生局科技基金(2014KP08)
天津市卫生行业重点攻关项目(16KG108)
中国肝炎防治基金会天晴肝病研究基金(TQGB20170123).
关键词
癌
肝细胞
NFATC转录因子类
免疫组化
微血管侵犯
Carcinoma, hepatocellular
NFATC transcription factors
Immunohistochemistry
Microvascular invasion
