摘要
背景:镁离子可通过自由基清除、细胞膜稳定、炎症抑制等作用降低血管内皮细胞对不利刺激的敏感性,调节血管内皮功能,但镁离子对内皮祖细胞的作用尚不清楚。目的:探讨镁对子痫前期患者内皮祖细胞增殖、黏附和凋亡的影响。方法:纳入2016年3月至2018年10月河北省人民医院产科17例子痫前期产妇与15名正常妊娠产妇,从两组产妇脐血中分离培养内皮祖细胞。将每例子痫前期产妇脐血来源内皮祖细胞分4组干预:子痫前期组以PBS干预,低、中、高浓度镁离子组分别以含4,8,16 mmol/L镁离子的培养基干预;正常妊娠产妇脐血来源内皮祖细胞以PBS干预,作为对照组。干预24 h后,检测各组细胞的增殖、黏附、凋亡、炎症因子(白细胞介素6、白细胞介素8、肿瘤坏死因子α与单核细胞趋化蛋白1)及氧化应激(超氧化物歧化酶、丙二醛)水平。实验方案已获河北省人民医院伦理委员会批准,伦理审批号:2013临审13号。结果与结论:①与对照组比较,子痫前期组细胞增殖与黏附数量明显降低(P <0.05),凋亡率升高(P <0.05),Bax和Active caspase-3蛋白表达升高(P <0.05),Bcl-2蛋白表达降低(P <0.05),白细胞介素6、白细胞介素8、肿瘤坏死因子α与单核细胞趋化蛋白1、丙二醛水平升高(P <0.05),超氧化物歧化酶活性降低(P <0.05);②与子痫前期组比较,镁离子干预各组细胞增殖与黏附数量升高(P <0.05),凋亡率降低(P <0.05),Bax和Active caspase-3蛋白表达降低(P <0.05),Bcl-2蛋白表达升高(P <0.05),炎症因子水平明显下降(P <0.05),超氧化物歧化酶活性升高(P <0.05),丙二醛水平降低(P <0.05),并且高浓度镁离子组改善程度优于低、中浓度镁离子组(P <0.05);③结果表明,镁离子可促进子痫前期患者体外内皮祖细胞的增殖和黏附,抑制炎性因子的分泌,降低凋亡率和氧化应激水平。
BACKGROUND:Magnesium ions can reduce the sensitivity of vascular endothelial cells to adverse stimuli and regulate vascular endothelial function by scavenging free radicals,stabilizing cell membranes and inhibiting inflammation.However,the effect of magnesium ions on endothelial progenitor cells is still unclear.OBJECTIVE:To explore the effects of magnesium on the proliferation,adhesion and apoptosis of endothelial progenitor cells in patients with preeclampsia.METHODS:From March 2016 to October 2018,17 preeclampsia pregnant patients and 15 normal pregnant women who were admitted in the Obstetrics Department of Hebei General Hospital were enrolled.Endothelial progenitor cells were isolated and cultured from umbilical cord blood in the two groups of pregnant women.Endothelial progenitor cells isolated from each preeclampsia pregnant patient were divided into four groups:preeclampsia group was intervened with PBS,and low,middle and high concentration magnesium ion groups were intervened with the medium containing 4,8,and 16 mmol/L magnesium ions,respectively.Endothelial progenitor cells from normal pregnant women were treated with PBS as control group.After 24 hours of intervention,cell proliferation,adhesion,apoptosis,inflammation(interleukin-6,interleukin-8,tumor necrosis factor-α,monocyte chemoattractant protein-1 levels)and oxidative stress(superoxide dismutase and malondialdehyde levels)were detected.The study protocol was approved by the Ethics Committee of Hebei General Hospital with the approval No.2013linshen13.RESULTS AND CONCLUSION:(1)Compared with the control group,the cell proliferation and adhesion of the preeclampsia group were significantly decreased(P<0.05);the apoptotic rate significantly increased(P<0.05);the expression of Bax and Active Caspase-3 increased,and the expression of Bcl-2 decreased(both P<0.05);the levels of interleukin-6,interleukin-8,tumor necrosis factor-α,monocyte chemoattractant protein-1,and malondialdehyde increased(P<0.05),and the activity of superoxide dismutase decreased(
作者
李丽
王新玲
刘素新
霍琰
Li Li;Wang Xinling;Liu Suxin;Huo Yan(Hebei General Hospital,Shijiazhuang 050051,Hebei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第21期3371-3377,共7页
Chinese Journal of Tissue Engineering Research
基金
河北省科技成果推广项目(20130102)
项目负责人:李丽~~
关键词
镁离子
子痫前期
脐血
内皮祖细胞
细胞增殖
细胞黏附
细胞凋亡
炎性因子
magnesium ion
preeclampsia
cord blood
endothelial progenitor cells
cell proliferation
cell adhesion
apoptosis
inflammatory factor
