摘要
目的建立一种简便的测定吸毒者尿液中毒品及代谢物的超高分辨液相色谱-质谱联用方法。方法样品不用特殊前处理,过尼龙6直径25mm,孔径0.22μm滤膜稀释后直接进样。色谱柱为Thermo Hypersil GOLD Aq 100mm×2.1mm,流动相为0.1%的甲酸水-甲醇,梯度洗脱,流速为0.2mL/min,柱温25℃,采用HESI离子源,正离子方式监测。结果甲基苯丙胺、吗啡、海洛因、可待因、氯胺酮、苯丙胺和单乙酰吗啡在1.0～200μg/L浓度范围内线性良好(r>0.9941)。方法回收率在96.5%～104.1%。日内和日间RSD均小于6%。结论本方法可靠简单、能快速检测尿液中毒品及其代谢物。
Objective To establish a simple and convenient method for determining the urine-contained drugs and metabolites by ultra-high resolution liquid chromatography-mass spectrometry. Methods Without special pretreatment, the sample was passed through a nylon filter (diameter of 25mm, pore size of 0.22μm), leaving the filtrate to inject into the apparatus directly. Thermo Hypersil Aq (100mm×2.1mm), the chromatographic column insetting into the Exactive Orbitrap LC/MS, was flowed at 0.2mL/min with the gradient-eluting mobile phase of 0.1% formic acid/hydro-methanol. The column temperature was set at 25℃, and HESI selected as the ion source monitored in the positive ion mode. Results Good linearity was shown from methamphetamine, morphine, codeine, heroin, ketamine, amphetamine and monoacetylmorphine in the range of 1.0~200μg/L (r>0.9941). The recovery was 96.5%~104.1%. Intra- and inter-day RSDs were less than 5.66%. Conclusion The method is simple and reliable, capable of rapid detection of urine-contained drugs and metabolites.
作者
崔军峰
许俊杰
刘瑶
李晨涛
CUI Junfeng;XU Junjie;LIU Yao;LI Chentao(Criminal Investigation Detachment of Baoji Public Security Bureau, Baoji 721006, Shaanxi, China)
出处
《刑事技术》
2019年第2期140-143,共4页
Forensic Science and Technology
关键词
毒品
代谢物
液-质联用
尿液
narcotics
metabolite
liquid-mass spectrometry
urine
