摘要
目的建立卵白蛋白(OVA)诱导家兔过敏性紫癜动物模型,为更进一步研究过敏性紫癜的发病机制提供理论依据。方法 20只家兔随机分两组:模型组和对照组(n=10)。模型组给予配方中药煎剂喂饮,1次/d,每次1 ml,连续3周,对照组给予等量生理盐水喂饮。模型组末次喂饮当日,腹腔注射乳化溶液1 ml,1次/周,连续3周。对照组给予等量生理盐水腹腔注射。末次腹腔注射7 d后,模型组兔耳缘静脉注射浓度为10 mg/ml的OVA生理盐水溶液0. 5ml,同时后背剃毛,并将浓度为0. 3%的OVA生理盐水皮内注射1 ml,分5点注射,每点0. 2 ml。对照组分别以相同的方法和剂量皮内注射生理盐水。注射后观察对比模型组和对照组动物的一般情况,24 h后检测实验室指标以及3周后检测皮肤、肾脏等组织的病理结果。结果模型组中6只家兔出现皮下紫斑,模型组与对照组在血液黏稠度、血常规及尿常规、Ig A、Ig G、C3、C4等指标方面差异有统计学意义。模型组家兔皮肤组织病理表现为真皮层水肿,毛细血管扩张、出血,炎症细胞浸润及血管壁的Ig A沉积。肾脏组织病理表现为肾小球系膜增厚、系膜细胞及基质增生、囊腔蛋白渗出及局灶性慢性肾炎伴大量免疫复合物沉积。结论配方中药煎剂喂饮、连续OVA刺激、静脉及皮内大剂量OVA冲击的方式,可成功模拟较可靠稳定的过敏性紫癜动物模型。
Objective To establish an animal model of allergic purpura induced by ovalbumin(OVA)in rabbits,and to provide a more suitable experimental model for further study of allergic purpura.Methods 20 rabbits were randomly divided into two groups:model group and control group(n=10).The model group was given the prescription traditional Chinese medicine decoction to drink once a day,1 ml each time for 3 weeks.The control group was given the same amount of physiological saline.In the model group,1 ml of emulsion solution was injected intraperitoneally on the day of the last feeding for 3 weeks.The control group was given the same amount of physiological saline intraperitoneal injection.7 days after the last intraperitoneal injection,model group rabbits were injected through the auricular vein with 10 mg/ml OVA saline solution of 0.5 ml.At the same time,shaving at the back and intradermal injection of 0.3%OVA saline 1 ml at 5 points.The control group was given intradermal injection of physiological saline with the same method and dose.Observation and comparison of the general situation of the two groups after injection,laboratory indexes were detected after 24 h,and pathological results of skin and kidney tissues after3 week were detected.Results Subcutaneous purple spots in 6 rabbits of the model group,there were significant differences between these two groups in terms of blood viscosity,blood and urine routine IgA,IgG,C3,C4.The pathological features of the skin in the model group were edema of the dermis,capillary dilatation,hemorrhage,inflammatory cell infiltration and IgA deposition in the vascular wall.Cortical edema,capillary dilatation,bleeding,inflammatory cell infiltration and IgA deposition in vascular walls.Renal histopathological features were glomerular mesangial thickening,mesangial cell and matrix proliferation,cystic protein exudation and focal chronic glomerulonephritis with a large amount of immune complex deposition.Conclusion A stable allergic purpura animal model can be successfully simulated by using
作者
侯兆玉
张晓翠
张红利
邓芳
Hou Zhaoyu;Zhang Xiaocui;Zhang Hongli(Dept of Paediatrics,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)
出处
《安徽医科大学学报》
CAS
北大核心
2019年第4期589-593,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省公益性技术应用研究联动计划项目(编号:1704f0804027)
安徽省教育厅高校优秀拔尖人才培育项目(编号:gxbjZD07)
关键词
过敏性紫癜
兔模型
病理
Henoch-Schonlein purpura
rabbit model
pathology
