摘要
医院感染暴发是患者安全的严重威胁,常常导致严重的后果。使用基因分型的方法明确暴发事件中的感染源和传播途径是控制感染暴发的重要因素。近年来,将病原体的全基因组测序(whole-genome sequencing,WGS)作为基因分型的工具,使得该项工作变得更加快速和精确。使用WGS技术能区分高度相关的病原体谱系,在确定暴发的同时追踪感染源信息,同时能提供更多的目标干预信息以及帮助理解传播动力学模型,避免了传统的同源性鉴别方法分型不精细、速度慢等缺陷。该文综述了WGS技术在医院感染暴发调查和控制中的应用实践,评价了其优势和面临的挑战。
Healthcare-associated infection outbreaks are a serious threat to patient safety and often cause serious consequences. The use of genotyping methods to identify the source of infection and the route of transmission in outbreaks is a critical point in controlling outbreaks. Recently, the use of whole-genome sequencing(WGS) makes it faster and much more accurate. Compared with traditional methods, WGS can distinguish highly correlated pathogen lineages,track infection source accurately and help researchers understanding the propagation dynamics model, and even provide more target intervention information. The application of WGS technology in healthcare-associated infection outbreak investigation and control is reviewed in this paper, and its advantages and challenges are also evaluated.
作者
乔甫
QIAO Fu(Infection Prevention & Control Department, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China)
出处
《华西医学》
CAS
2019年第3期334-337,共4页
West China Medical Journal
基金
四川省科技厅计划项目(2017ZR0170)
关键词
全基因组测序
医院感染
暴发
追踪
Whole-genome sequencing
Healthcare-associated infection
Outbreak
Tracking
