摘要
【目的】紫茎泽兰的入侵将对入侵地生态环境和农业经济造成严重危害,比较紫茎泽兰不同入侵区域土壤细菌多样性,丰富入侵植物的土壤微生物假说,也为紫茎泽兰的防控提供重要的理论依据。【方法】采用磷脂脂肪酸(PLFAs)分析法和16s rDNA高通量测序法,对不同紫茎泽兰重度入侵区域其根际土壤细菌群落进行分析比较,在此基础上使用相关性分析和主成分分析等统计方法,比较土壤细菌群落差异,探究其与土壤环境因子之间的相互关系。【结果】(1)各采样点中紫茎泽兰根际土壤细菌含量占微生物总量的比例均高于60%。其中Y2样点土壤细菌含量显著高于其他样点样品,达123.74μg·g^(-1),但该样地中检测到的门、科、属数量均低于其他样点。(2)从门水平上看,各样点土壤样品中相对丰度最高的前五个门分别是变形杆菌门、放线菌门、酸杆菌门、拟杆菌门和疣微菌门,其中变形杆菌门的相对丰度均为最高,在细菌群落中所占比例均超过30%;从属水平上看,四个样点存在不同的优势菌群,Y1样点优势菌群为固氮菌属和芽孢杆菌属,Y2样点优势菌群为乳酸杆菌属、结核分枝杆菌属以及根瘤菌属等,Y3样点优势属为鞘氨醇单胞菌属,Y4中的优势菌群为假单胞菌属。(3)土壤速效钾、铵态氮、有机碳、蔗糖酶、脲酶以及蛋白酶等环境因子与土壤细菌群落Alpha多样性指数存在显著相关性;土壤速效钾、硝态氮、有机碳、蔗糖酶、脲酶、蛋白酶等环境因子影响不同样点紫茎泽兰根际土壤细菌群落组成。【结论】不同紫茎泽兰重度入侵区域其根际土壤细菌在含量、门水平和属水平上均存在差异。土壤速效钾、有机质含量、蔗糖酶、脲酶等是影响土壤细菌群落结构的主要因素。
【Aim】 The alien plant Ageratina adenophora can do serious damage to the environment and agriculture production. Comparative study on soil bacterial community diversity in different invasive regions of A. adenophora greatly enriched the soil microbial hypothesis of invasive plants and provided an important theoretical basis for the prevention and control of A. adenophora.【Method】Phospholipid fatty acid (PLFAs) analysis and 16s rDNA high-throughput sequencing were used to analyze and compare the bacterial communities in the rhizosphere soil of different severely invasive areas of A. adenophora . Based on this, correlation analysis and principal component analysis were used to explore the relationship between soil bacterial community and soil environmental factors.【Result】(1) The ratio of bacterial content in the rhizosphere soil of A. adenophora to the total amount of microorganisms in each sampling area was higher than 60%. The soil bacterial concentration in Y2 was significantly higher than other samples, reaching 123.74 μg·g -1 , but the number of phyla, families and genera detected in this area was lower than other sample areas.(2) At the level of the phylum, the top five phyla with the highest relative abundance in all soil samples are Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, and Verrucomicrobia. In all soil samples, the relative abundance of Proteobacteria is the highest, whose proportion in the bacterial community is more than 30%. At the genus level, the four sample areas have different advantage genera. The dominant groups in Y1 are Pirellula and Bacillus, and the dominant bacteria in Y2 are Lactobacillus, Mycobacterum and Bradyrhizobium, the dominant bacterium in Y3 and Y4 is Sphingomonas and Psedomona respectively.(3) The environmental factors such as soil available potassium, ammonium nitrogen, organic carbon, sucrase, urease and protease have significant correlation with soil bacterial community Alpha diversity index;environmental factors such as soil available potass
作者
柳旭
孔令杰
杨康
韩月龙
张风娟
LIU Xu;KONG Lingjie;YANG Kang;HAN Yuelong;ZHANG Fengjuan(College of Life and Science, Hebei University, Baoding, Hebei 071002, China)
出处
《生物安全学报》
CSCD
2019年第1期49-58,共10页
Journal of biosafety
基金
国家自然科学基金项目(31372000)
河北省自然科学基金项目(C2015201021
C2015201241)
教育厅自然科学基金项目(ZD2016039)
关键词
紫茎泽兰
不同入侵区域
磷脂脂肪酸
高通量测序
土壤细菌
Ageratina adenophorum
different invasion area
phospholipid fatty acid
high-throughput sequencing
soil bacterial community
