摘要
采用不同浓度的m(吲哚丁酸)∶m(萘乙酸)=1∶1溶液浸泡洒金柏1年生半木质化插穗,研究各处理对洒金柏1年生半木质化插穗扦插的影响,并采用优化的石蜡切片技术研究不定根发育过程中插穗的外部形态及组织结构变化。结果表明:80 mg/L的m_(吲哚丁酸):m_(萘乙酸)=1∶1溶液浸泡4 h处理的插穗的生根率最佳,为91.38%。洒金柏扦插不定根形成过程可划分为愈伤形成期、诱导期、表达期、伸长期4个连续时期,历时90 d左右。洒金柏不定根原基属于诱生根原基,扦插前插穗内部没有潜伏根原基;愈伤组织是不定根形成、生长和发育的基础,必须经过诱导才能形成,属于愈伤诱导生根型。不定根原基来源于愈伤组织和髓射线与形成层交叉处,属于多位点发生。
One-year-old semi-lignified cutting of Platycladus orientalis were immersed in different concentrations of mIBA∶ mNAA=1∶1, and the effects of each treatment on 1-year-old semi-lignified cutting of P. orientalis were studied. Improved paraffin section technique was used to study the changes in the external morphology and tissue structure of the cuttings during the development of adventitious roots, which will lay the foundation for the study of the cytological mechanism of adventitious root development. The results show that the best rooting rate of cuttings treat with 80 mg/L mIBA∶mNAA =1∶1 for 4 h is 91.38%. Morphological observations reveals that the formation process of adventitious roots could consist of 4 consecutive periods of callus formation,induction, expression, and elongation, which lasts for about 90 days. Anatomical observations show that the root primordium belongs to the induced root primordium. There are no latent roots in the cuttings before cuttings. And callus forms the basis for the formation, growth and development of adventitious roots. It must be induced to form callus before rooting, and belongs to callus induction type. The adventitious root primordium originated from the intersection of the callus and the myelin rays with the formation layer, belonging to multiple sites to take root.
作者
赵今哲
刘国彬
张鸿翎
曹均
张玉萍
廖婷
王烨
郭丽琴
Zhao Jinzhe;Liu Guobin;Zhang Hongling;Cao Jun;Zhang Yuping;Liao Ting;Wang Ye;Guo Liqin(College Forestry, Inner Mongolia Agricultural University, Hohliot Inner Mongolia 010018, China;Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2019年第2期173-178,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
北京园林绿化增彩延绿科技创新工程(CEG-2017-01-04)资助
北京市农林科学院科技创新能力建设专项(KJCX20170111-08)资助
北京市林业果树科学研究院科技创新团队(JWKST201609)资助
关键词
洒金柏
扦插
不定根
外部形态
组织结构
吲哚丁酸
萘乙酸
Platycladus orientalis
cutting
adventitious root
external morphology
organization structure
IBA
NAA
