摘要
为研究海洋经济贝类在捕后面临无氧胁迫时的风味品质变化,从Opine脱氢酶(OpDHs)在无氧代谢中发挥的作用、OpDHs分布的物种与组织特异性、海洋经济贝类在环境胁迫下的OpDHs应答表现、OpDHs基本酶学性质4个方面对无脊椎动物无氧代谢中发挥重要作用的OpDHs进行了系统综述。无脊椎动物OpDHs主要包括Alanopine(ADH)、Octopine(ODH)、Strombine(SDH)、Tauropine(TDH)、β-Alanopine(BADH)和Lysopine(LyDH) 6种脱氧酶,在贝类中ODH活性最高;在甲壳类动物中几乎未发现ODH活性;在环节动物中只有少数动物存在ODH且活性较低,ADH和SDH起主要作用,肌肉组织中OpDHs活性最高;不同来源的OpDHs在基本酶学性质上均表现出相同或相似的性质。OpDHs具有作为活品代谢状态指标的潜力,可为捕后活品品质评价提供新思路。
The Opine dehydrogenase(OpDHs)is involved in regulation of anaerobic metabolism influencing flavor quality of marine commercial mollusks exposed to hypoxia or anoxia stress during the postharvest process.This article reviews the followings of the OpDHs:role in anaerobic metabolism,species-specific and tissue-specific distribution,OpDHs activity of different species in response to the environmental stress,and the basic enzymatic property of OpDHs in marine invertebrates,especially with the maximal activity of octopine dehydrogenase(ODH)in marine mollusks and without activity of ODH in crustacean.The high activities of alanopine dehydrogenase and strombine dehydrogenase were observed in a few annelids,with low ODH activity,and the maxiaml OpDHs activity in muscle.The OpDHs from different species showed the same or similar enzymatic properties,and have the possibility to become a potential indicator of physiological state,and to provide a new method for the quality evaluation of postharvested live products.
作者
郑尧
田元勇
刘洋
刘俊荣
ZHENG Yao;TIAN Yuan-yong;LIU Yang;LIU Jun-rong(College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2019年第2期296-302,共7页
Journal of Dalian Ocean University
基金
国家自然科学基金资助项目(31671790)
国家重点研发计划资助-蓝色粮仓科技创新(2018YFD0901001)
关键词
无氧代谢
Opine脱氢酶
海洋经济贝类
anaerobic metabolism
Opine dehydrogenase
marine commercial mollusk
