摘要
目的研究顺铂同步X线照射对人食管鳞状细胞癌Eca 109细胞增殖的影响及其机制。方法通过四甲基偶氮瞠盐(MTT )法检测单纯顺铂组细胞接受顺铂处理24 h的10%抑制浓度(IC10)及半数抑制浓度(IC50);单纯照射组接受4Gy 6 MV-X线照射,顺铂同步照射组细胞接受4Gy 6 MV-X线照射及IC10或IC50顺铂处理,培养24,36、48 h后MTT法检测细胞生长抑制情况。流式细胞术检测各组细胞周期及细胞凋亡情况。结果顺铂作用24h的Ecal09细胞IC10和IC50分别为0.894μg/ml和8.654μg/ml,培养24、36、48h后,4Gy照射+IC10顺铂组细胞抑制率分别为(26.1 ± 12)%、(56.4 ± 4.0)%、(75.1 ±3.2)%,4Gy 照射+ IC50顺铂组分别为(55.4 ±5.9 )%、( 84.7 ±3.1 )%、( 93.8 ±5.1)%,单纯照射组分别为(5.1 ±2.6 )%、( 12.2 ± 1.3 )%、( 37.9 ±5.3 )%。随着培养时间延长,三组细胞抑制率均逐渐上升,差异均有统计学意义(均P<0.01 );顺铂同步照射组细胞抑制率均高于单纯照射组,差异均有统计学意义(均P < 0.01 )。培养48 h后,空白对照组、单纯照射组、4Gy照射+ IC10顺铂组、4Gy照射+ IC50顺铂组G2 / M期细胞比例依次增高,分别为(3.0 ± 1.5 )%、( 10.4 ± 0.8 )%、( 24.8 ±3.1)%,(38.9 ± 1.2)%,组间差异有统计学意义(F= 224.3,P<0.01 );4Gy照射+IC10顺铂组、4Gy照射+ IC50顺铂组S期细胞比例分别为(23.4 ±7.7 )%、( 23.2 ± 5.2 )%,均低于空白对照组的(44.5 ±2.0)%,组间差异均有统计学意义(均p< 0.05 )。4Gy照射+ IC10顺铂组.4Gy照射+ IC50顺铂组细胞凋亡率分别为(14.0 ±4.2)%,( 17.9 + 3.0)%,均高于空白对照组的(4.6±1.8)%,差异均有统计学意义(均P<0.05 );4Gy照射+IC50顺铂组细胞凋亡率亦高于单纯照射组的(7.1 ±0.9)%,差异有统计学意义(P =0.001 )。结论顺铂同步X线照射对人食管鳞状细胞癌Eeal09细胞有明显的增殖抑制作用,并具有剂量和时间依赖性;其机制可能与小剂量或大剂量顺铂联合X线使细胞周期停滞于G2/M期.导致细胞对�
Objective To investigate the effect of cisplatin combined with X-ray irradiation on the proliferation of human esophageal squamous cell cancer Ecal09 cells and its mechanism. Methods Methyl thiazolyl tetrazolium (MTT) method was used to determine 10% inhibitory concentration (IC10) and half inhibitory concentration (IC50) of Ecal09 cells after 24 h treated with cisplatin in cisplatin group. Then the irradiation group was treated by 4 Gy 6 MV-X ray, cisplatin combined with X-ray irradiation group was treated with 4 Gy 6 MV-X ray iiTadiation, and IC10 or IC50 cisplatin treatment. After 24 h, 36 h and 48 h of culture, MTT method was used to detect the cell growth and inhibition in each group. Flow cytometry was used to detect cell cycle and apoptosis in each group. Results After 24 h for cisplatin treatment, IC10 and IC50 of Ecal09 cells was 0.894 μg/ml and 8.654 μg/ml. After 24 h, 36 h and 48 h of culture, the inhibition rates of 4 Gy+IC10 group were (26.1 ± 1.2)%,(56.4±4.0)% and (75.1 ±3.2)%, respectively, and 4 Gy+50 group were (55.4±5.9)%,(84.7±3.1)% and (93.8±5.1)%, respectively, and irradiation group were (5.1 ±2.6)%,(12.2±1.3)% and (37.9±5.3)%, respectively. With the prolonged culture time, the inhibition rates of the three groups were increased gradually, and the differences were statistically significant (all P<0.01);the inhibition rate of cisplatin combined with X-ray irradiation group was higher than that of irradiation group, and the differences were statistically significant (all P<0.01). After 48 h of culture, the proportion of G2/M phase cells in the blank control group, irradiation group, 4 Gy+IC10 group, and 4 Gy+IC50 group was increased to (3.0±1.5)%,(10.4±0.8)%,(24.8±3.1)% and (38.9±1.2)%, and the differences were statistically significant (F=224.3, P<0.001);the proportion of S phase cells in 4 Gy+ IC10 group and 4 Gy+IC50 group was (23.4±7.7)% and (23.2± 5.2)% respectively, which were lower than that in the blank control group [(44.5±2.0)%], and the differences were statisticall
作者
达春丽
刘凯
王若峥
Da Chunli;Liu Kai;Wang Ruozheng(Department of Radiotherapy for Head and Neck, the Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China)
出处
《肿瘤研究与临床》
CAS
2019年第2期78-83,共6页
Cancer Research and Clinic
基金
新疆维吾尔自治区自然科学基金(20152IIC115).
关键词
食管肿瘤
抗肿瘤联合化疗方案
放射疗法
细胞凋亡
细胞周期
Esophageal neoplasms
Antineoplastic combined chemotherapy protocols
Radiotherapy
Apoptosis
Cell cycle
