摘要
种质资源遗传多样性和群体结构分析为复杂性状关联分析提供基础。本研究通过SSR分子标记技术,对68份柱花草(Stylosanthes spp.)种质资源的遗传多样性与群体结构进行分析。结果表明,30个多态性SSR标记在68份柱花草种质中共检测到146个等位基因,平均为4.867个,每个SSR标记的Shannon信息指数(I)和多态性信息含量(PIC)分别为0.676～1.690和0.363～0.764,平均为1.195和0.577。根据SSR标记数据进行遗传多样性分析,可将68份柱花草种质资源分为6类,群体结构分析中在ΔK达到最大时,K=6,因此将68份柱花草材料划分为6个群体,与遗传多样性分析结果基本一致。本研究将为柱花草遗传背景研究、种质资源评价和优良种质筛选提供依据。
Correlation analysis of complex traits is based on the genetic diversity and population structure analysis of germplasm resources. In this paper, genetic diversity and population structure of 68 Stylosanthes materials were analyzed using SSR molecular markers. The results showed that a total of 146 alleles were detected in the 68 germplasm materials of 30 polymorphic markers, with an average of 4.867. The Shannon information index (I) and polymorphism information content (PIC) of each SSR marker was 0.676~1.690 and 0.363~0.764, respectively, with an average of 1.195 and 0.577. According to the genetic diversity analysis based on SSR marker data, 68 germplasm resources of Stylosanthes can be divided into six clusters. In the analysis of population structure, when ΔK reached a maximum, i.e., K = 6 , the 68 Stylosanthes accessions were divided into six groups. Our result was consistent with the results of the genetic diversity analysis. This study could provide a basis for correlation analysis and for mining elite genes of Stylosanthes.
作者
卞华
申晴
韦海燕
蒋亚君
丁西朋
白昌军
BIAN Hua;SHEN Qing;WEI Haiyan;JIANG Yajun;DING Xipeng;BAI Changjun(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,Hainan,China;Tropical Crops Genetic Resources Institute,CATAS,Danzhou 571737,Hainan,China)
出处
《草业科学》
CAS
CSCD
2019年第3期729-743,共15页
Pratacultural Science
基金
国家自然科学基青年基金项目(31602000)
国家自然科学基金国际合作与交流项目(31861143013)
中国热带农业科学院基本科研业务费专项资金(1630032017006)
关键词
柱花草
种质资源
SSR标记
遗传多样性
群体结构
Stylosanthes
germplasm resources
SSR markers
genetic diversity
population structure
