摘要
目的探讨白细胞介素28B(IL-28B)基因单核苷酸多态性(SNP)及基因表达水平与宫颈癌的关系。方法随机选取低级别鳞状上皮内病变(LSIL组)、高级别鳞状上皮内病变(HSIL组)及宫颈癌术前(术前组)、术后(术后组)患者各30例,并选取成年健康体检女性30例作为对照组。取各组外周静脉血,采用PCR技术扩增IL-28B (rs12979860、rs8099917)的特异性片段,扩增产物直接测序分析其基因型、等位基因;应用RT-qPCR法测定外周血IL-28B mRNA。结果 IL-28B rs12979860、rs8099917位点基因型、基因频率在各组间分布差异无统计学意义(P均>0.05)。LSIL组、HSIL组、术前组及术后组外周血IL-28B mRNA水平均低于对照组(P均<0.05);宫颈癌患者中,术后组IL-28B mRNA水平高于术前组(P<0.05),术前组及术后组无淋巴结转移者外周血IL-28B mRNA水平均高于淋巴结转移者(P均<0.05)。结论 IL-28B基因rs12979860、rs8099917位点的SNP可能与宫颈癌发病无关,而IL-28B mRNA低水平表达在宫颈癌的发生发展中可能发挥重要作用。
Objective To investigate the single nucleotide polymorphisms (SNPs) of interleukin-28B (IL-28B) and the relationship between its expression level and cervical cancer.Methods Peripheral venous blood was taken from 30 patients with low-grade squamous intraepithelial lesion (LSIL group), 30 patients with high-grade squamous intraepithelial lesion (HSIL group), 30 patients with cervical cancer before surgery (preoperative group), 30 patients with cervical cancer after surgery (postoperative group), and 30 healthy adult women (control group). The specific fragments of IL-28B (rs12979860 and rs8099917) were amplified by PCR, and the amplified products were sequenced directly. IL-28B mRNA level in the peripheral blood was determined by RT-qPCR.Results The genotypes and gene frequencies of IL-28B rs12979860 and rs8099917 were not significantly different between these groups (all P >0.05). The expression of IL-28B mRNA in the LSIL group, HSIL group, preoperative group, and postoperative group was lower than that of the control group (all P <0.05). The expression of IL-28B mRNA in the postoperative group was higher than that in the preoperative group ( P <0.05). The expression of IL-28B mRNA in the cervical cancer patients without lymphatic metastasis was higher than that in patients with lymphatic metastasis ( P <0.05). Conclusion The SNPs of IL-28B (rs12979860 and rs8099917) may be not related to the incidence of cervical cancer, and the low expression of IL-28B mRNA may play an important role in the occurrence and development of cervical cancer.
作者
李雯
甄洁玉
周利军
苟元凤
韩雪
刘会玲
LI Wen;ZHEN Jieyu;ZHOU Lijun;GOU Yuanfeng;HAN Xue;LIU Huiling(Gansu Provincial People's Hospital, Lanzhou 730000, China)
出处
《山东医药》
CAS
2019年第8期21-24,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81560426)
甘肃省人民医院院内科研基金项目(16GSSY6-8)
关键词
宫颈癌
白细胞介素28B
单核苷酸多态性
低级别上皮内病变
高级别上皮内病变
cervical carcinoma
interleukin-28B
single nucleotide polymorphism
low-grade squamous intraepithelial lesions
high-grade squamous intraepithelial lesions
