摘要
目的探讨抗核抗体谱(anti nuclear antibody spectrum,ANAs)在类风湿性关节炎患者中的检测意义。方法选取我院2015年9月至2017年9月间康复免疫科收治的类风湿性关节炎患者103例作为研究对象(研究组);另选取我院2015年9月至2017年9月期间的健康体检者120例作为对照组。采用间接免疫荧光法检测ANA,采用免疫印迹法检测ANAs。比较两组ANA阳性情况,类风湿性关节炎ANA阳性核型分布情况,及两组ANAs各项阳性情况。结果研究组ANA阳性率(60.19%)明显高于对照组(5.00%),且有统计学差异(P<0. 05)。类风湿性关节炎ANA阳性核型分布中,以胞浆颗粒型、核均质型和核颗粒型为主。类风湿性关节炎患者103例中,ANAs阳性71例,阳性率为68.93%;对照组80例中,ANAs阳性0例,阳性率为0.00%。类风湿性关节炎患者中,以抗Ro-52、抗SS-A和抗SS-B为主。研究组抗Ro-52、抗SS-A、抗SS-B、抗nRNP和抗dsDNA阳性率高于对照组(P <0. 05);研究组抗核小体、抗CENPB、抗Sm、抗Scl-70、抗Jo-1、抗Histone和抗PCNA阳性率高于对照组(P>0. 05)。结论 ANAs检测在类风湿性关节炎患者中具有重要价值,能够明确患者血清中自身抗体种类,具有重要临床研究意义。
Objective To explore the detection significance of anti nuclear antibody spectrum (ANAs)in patients with rheumatoid arthritis. Methods A total of 103 patients with rheumatoid arthritis admitted to our hospital Rehabilitation Immunology Department from September 2015 to September 2017 were selected as research subjects (Study Group),and another 120 healthy subjects from September 2015 to September 2017 were selected as control group. ANA was detected by indirect immunofluorescence and ANAs was detected by Western blotting. The positive status of the two groups of ANA,the distribution of ANA positive karyotype in rheumatoid arthritis and the positive conditions of the two groups of ANAs were compared. Results The positive rate of ANA (60.19%)in the study group was significantly higher than that of the control group (5.00%)( P<0.05 ). The distribution of ANA positive karyotype in rheumatoid arthritis was dominated by cytoplasmic granulotyping,nuclear homogenization and nuclear granulotyping. Of the 103 patients with rheumatoid arthritis,71 cases of ANAs positive were positive,the positive rate was 68.93%;in the control group,0 cases of ANAs positive were positive,the positive rate was 0%. In patients with rheumatoid arthritis,they are mainly anti Ro-52,anti SS-A and anti SS-B. The research group of anti Ro-52,anti SS-A,anti SS-B,anti nRNP and anti dsDNA positive rate was higher than the control group ( P<0.05 );the study group of anti nucleosome,anti CENPB,anti Sm,anti Scl-70,anti Jo-1,anti Histone and anti PCNA positive rate was higher than the control group ( P>0.05 ). Conclusion ANAs detection is of great value in rheumatoid arthritis patients. It can identify the types of autoantibodies in serum and has important clinical significance.
作者
张思荣
陈艳芝
ZHANG Si-rong;CHEN Yan-zhi(Department of Clinical Laboratory,Baoshan People′s Hospital,Yunnan Province,Baoshan 678000,China)
出处
《标记免疫分析与临床》
CAS
2019年第2期205-207,219,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家科技支撑计划(编号:2015BAI32H00)
关键词
抗核抗体谱
类风湿性关节炎
检测意义
Anti nuclear antibody spectrum
Rheumatoid arthritis
Detection significance
