摘要
从四川矿区泡菜样品中分离得到1株对重金属铅(Pb)、铬(Cr)和铜(Cu)具有较高耐受性的菌株,经16S rDNA初步鉴定为1株植物乳杆菌。研究重金属铅、铬和铜对该植物乳杆菌的最小抑制浓度(MIC)。比较不同初始pH、初始离子浓度、吸附时间和菌体加入量对植物乳杆菌吸附3种重金属的影响,探讨MIC与吸附作用相关性。使用MIC的方法测定重金属对该菌的最小抑制浓度,原子吸收法测定对重金属的吸附效果。研究表明,该菌对Pb^(2+)、Cr^(6+)和Cu^(2+)的耐受性分别为6.67、0.67和2.17 mmol/L;其吸附性最适初始pH分别为4、6和6;最优初始离子浓度分别为100、100和50 mg/L;最优加菌量分别为3、6和5 g/L;最佳吸附时间分别为12、2和8 h。在100 mg/L的初始离子浓度下对Pb^(2+)、Cr^(6+)和Cu^(2+)的吸附率最高分别可达96%、61%和49%。MIC与吸附作用没有明显相关性。结果表明该菌具有优良的吸附性能,为今后含有乳酸菌的食品或饲料制剂的开发提供了新的乳酸菌种。
A strain highly possessing tolerance to heavy metals, lead, chromium and copper, was isolated from pickles samples in Sichuan mining area. It was identified initially as Lactobacillus plantarum by 16S rDNA. The minimal inhibitory concentration (MIC) of lead, chromium and copper against L. plantarum was studied. The effects of different initial pH, initial ion concentration, adsorption time and bacterial adding volume on the adsorption of three heavy metals by L. plantarum were compared, and the relativity of MIC and adsorption were approached. The MIC method was used to determine the minimum inhibitory concentration of heavy metals against the bacteria. The adsorption of heavy metals was tested by atomic absorption spectrometry. The results showed that the tolerance of the L. plantarum to Pb 2+, Cr 6+ and Cu 2+ were 6.67,0.67 and 2.17 mmol/L,respectively.The initial optimum pH of adsorption is 4, 6 and 6, respectively. The optimal initial ionic concentration was 100,100 and 50 mg/L respectively, and the best adding volume of bacteria was 3, 6 and 5 g/L respectively, and the best adsorption time was 12, 2 and 8 h, respectively. The highest adsorption rate of Pb 2+, Cr 6+ and Cu 2+ could reach 96%, 61% and 49% under the initial ion concentration at 100 mg/L. There is no significant correlation between MIC and adsorption. The results showed that the bacteria had excellent adsorption properties and provided new lactic acid bacteria for the development of food or feed preparation containing lactic acid bacteria in the days to come.
作者
李冉
欧杰
代启虎
马晨晨
LI Ran;OU Jie;DAI Qi-hu;MA Chen-chen(Coll. of Food Sci. & Technol.,Shanghai Ocean Uni.,Shanghai 201306;Shanghai Aquatic & Storage Engin. Technol.Res. Ctr.,Shanghai 201306;Lab. of Qual. & Safety Risk Assessm′t for Aqua-Prod. onStorage & Preserv′n (Shanghai),Min. of Agric.,Shanghai 201306)
出处
《微生物学杂志》
CAS
CSCD
2019年第1期52-58,共7页
Journal of Microbiology
基金
国家自然科学基金项目(31671779)
关键词
植物乳杆菌
重金属
最小抑制浓度(MIC)
吸附
Lactobacillus plantarum
heavy metal
minimum inhibitory concentration(MIC)
adsorption
