摘要
目的观察不同质量分数京尼平交联活体正常兔角膜的有效性及对角膜基质细胞形态的影响。方法选取40只健康新西兰白兔的右眼为实验眼,采用随机数字表法随机分为0.20%京尼平组、0.25%京尼平组、核黄素组和正常对照组。交联前、交联后7d及14d时,活体测量实验眼的平均角膜曲率(Km)和中央角膜厚度(CCT)。角膜交联后7d和14d,各组分别取5只实验眼角膜进行单轴拉伸法测量应变为10%的角膜弹性模量和应力;使用普通光学显微镜和透射电子显微观察角膜基质细胞结构。结果各组交联前、交联后7d和14dKm均数的总体比较,差异均无统计学意义(F时间=1.287,P=0.284;F组别=0.301,P=0.825)。各组交联前、交联后7d和14dCCT均数的总体比较,差异有统计学意义(F时间=3.786,P=0.029),与交联前相比,各组交联后7dCCT均明显增加,差异均有统计学意义(均P<0.05);各组间CCT比较,差异无统计学意义(F分组=0.557,P=0.646)。交联后7d,0.20%京尼平组、0.25%京尼平组、核黄素组和正常对照组的角膜弹性模量依次为(11.96±5.74)、(21.24±6.77)、(18.76±3.34)和(11.56±4.37)MPa,应力依次为(0.68±0.24)、(1.20±0.25)、(1.01±0.30)和(0.69±0.26)MPa;其中0.25%京尼平组弹性模量和应力高于0.20%京尼平组和正常对照组,差异均有统计学意义(均P<0.05),核黄素组与0.25%京尼平组弹性模量和应力比较,差异均无统计学意义(均P>0.05)。交联后14d,0.20%京尼平组、0.25%京尼平组、核黄素组和正常对照组的弹性模量依次为(16.65±3.19)、(19.12±2.39)、(22.83±4.38)和(12.70±2.72)MPa,应力依次为(0.83±0.12)、(0.97±0.04)、(1.23±0.30)和(0.65±0.20)MPa;其中核黄素组弹性模量和应力高于0.20%京尼平和正常对照组,差异均有统计学意义(均P<0.05),核黄素组与0.25%京尼平组弹性模量比较,差异无统计学意义(P=0.090),核黄素组应力高于0.25%京尼平组,差异有统计学意义(P=0.046)。光学显微镜下0.20%�
Objective To evaluate the efficiency and safety of genipin collagen crosslinking(G-CXL)on rabbit corneas in vivo.Methods Forty healthy New Zealand white rabbits were randomly divided into 0.20% G-CXL,0.25% G-CXL,standard UVA-CXL and normal control group.And the right eyes were treated in different grouping.No procedures were performed in the normal control group.The corneal curvature(Km)and central corneal thickness(CCT)of right eyes were evaluated before,7 days and 14 days after crosslinking treatment.Cornea strips were harvested from the right eyes and tensile strain measurements were performed 7 days and 14 days after crosslinking treatment.The structure of corneal stroma was observed under light microscope(LM)and transmission electron microscope(TEM).Results No statistically significant differences in Km were observed among different groups or different timepoints(Fgroup=0.301,P=0.825;F time=1.287,P=0.284).Significant difference in CCTs was noticed among different time pionts(Ftime=3.786,P=0.029).Compared with preoperative,the CCTs of all the groups were significantly increased 7 days after crosslinking(all at P<0.05).No significant difference in CCT was found among the groups(F group=0.557,P=0.646).Seven days after crosslinking treatment,the Young's modulus at 10%strain was(11.96±5.74),(21.24±6.77),(18.76±3.34)and(11.56±4.37)MPa in 0.20% G-CXL group,0.25% G-CXL group,UVA-CXL group and normal control group,respectively;the stress at 10%strain was(0.68±0.24),(1.20±0.25),(1.01±0.30)and(0.69±0.26)MPa,respectively;the Young's modulus and stress in 0.25% G-CXL group was significantly increased when compared with those in 0.20% G-CXL and normal control group(both at P<0.05).No significant difference in Young's modulus and stress was observed between 0.25% G-CXL group and UVA-CXL group(all at P>0.05).Forteen days after crosslinking treatment,Young's modulus at 10%strain was(16.65±3.19),(19.12±2.39),(22.83±4.38)and(12.70±2.72)MPa in 0.20% G-CXL group,0.25% G-CXL group,UVA-CXL group and normal control grou
作者
汤韵
宋文静
乔静
荣蓓
李海丽
杨松霖
晏晓明
Tang Yun;Song Wenjing;Qiao Jing;Rong Bei;Li Haili;Yang Songlin;Yan Xiaoming(Department of Ophthalmology,Peking University First Hospital,Beijing 100034,China)
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2019年第3期164-170,共7页
Chinese Journal Of Experimental Ophthalmology
基金
国家自然科学基金项目(11372011)
北京市自然科学基金项目(7142159)
中国科学院力学研究所非线性力学国家重点实验室课题项目.
关键词
角膜交联
京尼平
生物力学
角膜基质细胞
透射电子显微镜
Corneal collagen crosslinking
Genipin
Biomechanics
Keratocyte
Transmission electron microscope
