摘要
抗菌药物的应用能对细菌感染性疾病进行有效地控制和治疗,但水产动物养殖中滥用和误用抗菌药物导致耐药细菌的产生、养殖水环境污染和养殖动物药物残留等一系列问题,应对和解决抗菌药物在水产动物养殖中的滥用问题已经刻不容缓。本研究通过抗菌药物杀菌、组织细菌清除、细菌攻毒、鱼体存活率统计和药物敏感性检测等方法,探究外源L-半胱氨酸添加对氟苯尼考杀菌作用的影响。体外杀菌结果显示,4.00 mmol/LL-半胱氨酸可使20.00～200.00μg/mL氟苯尼考对嗜水气单胞菌的杀菌效率提高1.9～11.1倍,其联合用药的杀菌作用具有剂量和时间依赖效应。组织细菌清除结果显示,相比单独使用氟苯尼考,L-半胱氨酸与氟苯尼考合剂对鱼体肝脏、脾脏和肾脏组织中嗜水气单胞菌的清除能力提高4～16倍。细菌攻毒后,采用注射和口服L-半胱氨酸或/和氟苯尼考进行治疗,结果发现L-半胱氨酸与氟苯尼考合剂可大幅提高黄河鲤对嗜水气单胞菌的抗感染能力。同时,单独添加L-半胱氨酸也能促进组织细菌的清除和鱼体的抗感染能力。细菌MIC测定结果显示,添加1.00～4.00 mmol/L L-半胱氨酸使嗜水气单胞菌对氟苯尼考的敏感性提高2～4倍。由此推测,L-半胱氨酸可能通过增强嗜水气单胞菌对氟苯尼考的敏感性,从而提高抗菌药物对鱼体细菌感染的防治效果。研究表明,L-半胱氨酸作为氟苯尼考的增效剂不仅可提高抗菌药物的防治效果,而且可大幅降低抗菌药物的使用量,对嗜水气单胞菌的防控具有一定的实际应用价值。
Antimicrobial agents play an important role in the prevention and treatment of bacterial infections.However,the extensive use of antimicrobial agents,particularly the misuse of antibiotics in the aquatic animal breeding,has led to various issues,such as antibiotic resistance,pollution of breeding environments,and presence of drug residues in livestock,which need to be addressed urgently.In this paper,the effects of exogenous Lcysteine on the susceptibility of Aeromonas hydrophila to florfenicol were studied.The in vitro bactericidal assay found that 4.00 mmol/L L-cysteine could significantly improve the bactericidal effect of florfenicol by 1.9-11.1 times and that the bactericidal effect increased with increasing L-cysteine concentration and exposure time.Tissue bacterial eradication experiments showed that the combination of L-cysteine and florfenicol could significantly eliminate A.hydrophila in liver,spleen and kidney tissue by 4-16 times compared to florfenicol alone.Oral administration or injection with L-cysteine or/and florfenicol showed that the combination of L-cysteine and florfenicol greatly increased the survival rate of infected fish.Moreover,we also found L-cysteine played a certain role in tissue bacteria eradication and fish immune protection.At last,we found that 1.00-4.00 mmol/L L-cysteine increased the susceptibility of A.hydrophila to florfenicol by 2-4 times.We speculate that L-cysteine may enhance the susceptibility of A.hydrophila to florfenicol and increase the bactericidal effect of florfenicol.In summary,Lcysteine as a synergistic agent of florfenicol,can not only improve the bactericidal effect,but also significantly reduce the use of antimicrobial agents.Therefore,we present a novel approach in fighting against A.hydrophila with practical application value.
作者
赵贤亮
靳朝晖
黄莎妮
陈鹤
李莉
孔祥会
ZHAO Xianliang;JIN Zhaohui;HUANG Shani;CHEN He;LI Li;KONG Xianghui(College of Fisheries,Henan Normal University,Xinxiang 453007,China;College of Life Science,Henan Normal University,Xinxiang 453007,China)
出处
《水产学报》
CAS
CSCD
北大核心
2019年第3期671-678,共8页
Journal of Fisheries of China
基金
国家自然科学基金(31502204)
河南省科技计划项目(172102310545)
广东省海洋生物技术重点实验室开放基金(GPKLMB201701)
河南师范大学优秀青年科学基金(5101229279105)
河南省水产动物免疫与疾病防控创新型科技团队(201706081)~~
