摘要
为建立一种高效液相色谱法定量分析质量分数40%苯醚甲环唑·氟硅唑微乳剂,采用C_(18)色谱柱,DAD紫外检测器,流动相为甲醇和水(体积比85∶15),流速为1 mL/min,检测波长220 nm,对试样中苯醚甲环唑和氟硅唑进行分离测定.结果表明:苯醚甲环唑和氟硅唑保留时间分别为6.320和4.700 min,当进样质量浓度为10～100 mg/L时,线性相关系数分别为0.999 8和0.999 9;标准偏差分别为0.027 0和0.023 9;变异系数分别为0.077 0%和0.479 0%;平均回收率分别为99.70%和99.11%.通过该方法苯醚甲环唑和氟硅唑分离较好,适用于制剂中有效成分的定量分析.
A high performance liquid chromatography method for the determination of difenoconazole·flusilazole 40% micro-emulsion was established.The separation and determination of difenoconazole·flusilazole in the samples were conducted by C 18 column with the mixture of methanol and water as mobile phase (85∶ 15,by vol) at the flow rate of 1 mL/min at 220 nm by DAD detector. The results showed that the retention time of difenoconazole and flusilazole are 6.320 and 4.700 min. At the injection concentration from 10~100 mg/L, the linear correlation coefficients for difenoconazole and flusilazole were 0.999 8 and 0.999 9, the standard deviations were 0.027 0 and 0.023 9, the variation coefficients were 0.077 0% and 0.479 0%, and the average recoveries were 99.70% and 99.11%, respectively. In this method, difenoconazole·flusilazole with good separation effect, was suitable for quantitative analysis of active ingredients in preparation.
作者
张蕊
程东美
ZHANG Rui;CHENG Dongmei(College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
出处
《仲恺农业工程学院学报》
CAS
2019年第1期5-8,共4页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东省现代花卉产业技术体系创新团队建设(2018LM1131)资助项目
