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层流洁净手术室术中人员流动量对空气中细菌数及切口感染的影响 被引量:13

Effects of the flow of people in the laminar flow clean operating room on the number of bacteria in the air and wound infection
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摘要 目的探讨层流洁净手术室术中人员流动量对空气中细菌数及切口感染的影响,为医院感染的防控提供参考。方法选取2017年1—12月期间在我院同一间千级层流洁净手术室内手术的130例手术患者为研究对象,将患者根据术中人员流动情况分为流动组98例和与非流动组32例,采用静态采样法在开始手术前(开启空气过滤装置30 min,但患者未进入手术室)、术中30,60,90,120 min时、术毕时(开始缝合切口时)对手术室内空气进行采样,后行细菌培养,观察及计算空气中细菌数,同时观察术后切口感染发生情况。结果两组术前空气中细菌数比较差异无统计学意义(P> 0. 05),术中30,60,90,120,术毕时流动组空气中细菌数明显高于非流动组,差异比较有统计学意义(P <0. 05)。流动组术中不同人员流动量术前空气中细菌数比较差异无统计学意义(P> 0. 05),术中30,60,90,120,术毕时,术中人员流动量> 10人次的空气中细菌数明显高于术中人员流动量≤10人次,差异比较有统计学意义(P <0. 05);流动组术后15例患者发生切口感染,非流动组术后未发生切口感染,差异比较有统计学意义(P <0. 05)。流动组中15例切口感染患者均为术中人员流动量> 10人次,术中人员流动量≤10人次未发生切口感染,差异比较有统计学意义(P <0. 05);术中人员流动量> 10人次时手术室内空气中细菌数及切口感染率明显增高,有正相关性(P <0. 05);术中人员流动量≤10人次时手术室内空气中细菌数明显增高,有正相关性(P <0. 05),但切口感染率未增加,无相关性(P> 0. 05)。结论术中人员流动量可明显影响到层流洁净手术室空气中细菌数量,人员流动量>10人次时空气中细菌数量的升高,导致术后切口感染发生风险性增高。 Objective To explore the influence of the flow of people in the laminar flow clean operating room on the number of bacteria in the air and wound infection, and provide reference for the prevention and control of nosocomial infection. Methods A total of 130 surgical patients who underwent surgery in the same class of laminar flow clean operation in our hospital from January 2017 to December 2017 were selected as the study subjects. They were divided into mobile group with 98 patients and non-mobile group with 32 patients according to the intraoperative flow of people. Static sampling method was used to collect samples of air in the operation room before starting surgery (opening the air filter device for 30 min, but the patient did not enter the operating room), at 30, 60, 90, and 120 minutes during the operation, and at the end of the operation (when the suture was started). After that, bacterial culture was conducted and the number of bacteria in the air was observed and calculated. Meanwhile, the incidence of postoperative wound infection was observed. Results There was no significant statistical difference in the number of bacteria in the air between the two groups( P >0.05). The number of bacteria in the air of the mobile group was significantly higher than that in the non-mobile group at 30, 60, 90, and 120 minutes during the operation and after the completion of operation,and the difference was statistically significant( P <0.05). There was no significant statistical difference in comparison of the number of bacteria in the air before the operation in the mobile group under conditions of different personnel flows( P >0.05). At 30, 60, 90, and 120 minutes during the operation, as well as at the end of operation, the number of bacteria in the air with the intraoperative flow > 10 people was significantly higher than that in the operation room with the intraoperative flow ≤10 people,and the difference was statistically significant( P <0.05). A total of 15 patients in the mobile group had wound infection, and
作者 张颖 ZHANG Ying
出处 《护理实践与研究》 2019年第4期115-118,共4页 Nursing Practice and Research
关键词 层流洁净手术室 人员流动 细菌 切口感染 Laminar flow clean operating room Personnel flow Bacteria Incision infection
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