摘要
为研究牛源cathelicidin类抗菌肽的生物学功能,通过生物信息学方法对牛源cathelicidin类抗菌肽的理化性质、信号肽、跨膜区、亚细胞定位、二级结构和化学修饰等进行预测分析.结果表明:牛源cathelicidin类抗菌肽为阳离子型抗菌肽,等电点11.53～13.20;除了BMAP-27和Dodecapeptide为稳定的多肽外,其他抗菌肽均为不稳定多肽;BMAP-28和Dodecapeptide为疏水性多肽,BMAP-27、BMAP-34、Bac-4、Bac-5、Bac-7和Indolicidin为亲水性多肽;BMAP-27和BMAP-28由α螺旋、β折叠、β转角和无规则卷曲构成,BMAP-34主要由α螺旋和β转角构成,Bac4、Bac7、Indolicidin和Dodecapeptide由无规则卷曲构成,Bac5由β转角和无规则卷曲构成;没有信号肽、跨膜区和糖基化位点,BMAP-27、BMAP-28和BMAP-34各存在1个丝氨酸的磷酸化位点.根据分析结果推测牛源cathelicidin类抗菌肽可作用于细菌的细胞壁或细胞膜,进而导致细菌死亡.
To study the biological functions of bovine cathelicidin antimicrobial peptides(bcAMPs),tools on line were applied to analyze the physical and chemical properties,signal peptide,trans-membrane region,subcellular localization,secondary structure and chemical modification of bcAMPs.The results showed that bcAMPs are cationic antimicrobial peptide,and their isoelectric point are between 11.53 and 13.20.Except that BMAP-27 and Dodecapeptide are stable peptide,other bcAMPs are unstable peptide.BMAP-28 and Dodecapeptide are hydrophobic peptide,while BMAP-27,BMAP-34,Bac-4,Bac-5,Bac-7 and Indolicidin are hydrophilic peptide.BMAP-27 and BMAP-28 have alpha helix,beta sheet,beta turn and random curl,while BMAP-34 is mainly composed of alpha helix and beta turn.Bac4,Bac7,Indolicidin and Dodecapeptide consist of random curl.Bac5 consists of beta sheet and random curl.The bcAMPs have no signal peptide,trans-membrane region and glycosylation sites.BMAP-27,BMAP-28 and BMAP-34 have only one serine phosphorylation site respectively.These findings suggested that bcAMPs could kill bacterial through the interaction with cell wall or cell membrane.
作者
李杰
杭柏林
宁春妹
司素锦
张慧辉
胡建和
LI Jie;HANG Bolin;NING Chunmei;SI Sujin;ZHANG Huihui;HU Jianhe(Agriculture and Rural Bureau of Xinxiang in Henan Province,Xinxiang 453003,China;School of Animal Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《河南科技学院学报（自然科学版）》
2019年第1期37-42,共6页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
河南省自然科学基金项目(182300410031)
河南科技学院高层次人才启动项目(2014020)
河南科技学院大学生创新训练项目(2018CX88)
河南科技学院大学生"百农英才"创新项目(BNYC2017-2-17)
