摘要
[目的]为提高优质奶牛的繁殖力,保证低成本的情况下大量生产优质胚胎,对荷斯坦奶牛采用活体采卵的方式采集卵母细胞。[方法]将4头荷斯坦奶牛分为超排组和非超排组2组,超排组对试验牛注射FSH,非超排组对对照牛不采取任何处理。对荷斯坦奶牛进行活体采卵,并对卵母细胞数进行统计。[结果]共采集到12枚卵母细胞,经过受精培养后有8枚受精卵发育良好。[结论]采用优化的活体采卵程序并配合超排技术,能够更好地利用良种奶牛,以达到动物胚胎生产和辅助生殖的目的。
[Objective]To improve the fecundity of high-quality breed cattle and produce mass of high-quality embryo with low cost,ovum was collected from Holstein dairy cows by using ovum pick-up technology.[Method]Four Holstein dairy cows were divided into two groups:superovulation group(A) and non-superovulation group(B).The dairy cows in group A was injected with FSH,no treatment was made on dairy cows in group B.The ovum was collected from Holstein dairy cows by using B ultrasonic instrument and statistics on the number of collected ovum was made.[Result]12 oocytes were obtained,and 8 fertilized eggs developed well after fertilization culture.[Conclusion]This experiment adopted an optimized ovum pick-up procedure combined with superovulation technology,which can make better use of improved dairy cows and achieve the production of animal embryo and assisted reproduction.
作者
黄启震
卢文瑾
王丽娟
谢彤彤
付博凡
袁梦怡
王相国
HUANG Qi-zhen;LU Wen-jin;WANG Li-juan(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206)
出处
《安徽农业科学》
CAS
2019年第4期107-109,共3页
Journal of Anhui Agricultural Sciences
基金
国家重点研究计划项目(2017YFD0502200)
北京市奶牛产业创新团队项目(BAIC05-2017)
关键词
活体采卵
荷斯坦奶牛
体外受精
Ovum pick-up(OPU)
Holstein dairy cows
In vitro fertilization(IVF)
