摘要
【目的】分析外源生长激素对水稻T-DNA插入突变体褐飞虱抗性的影响,并测定其农艺性状,为突变基因介导的抗虫分子机理研究提供理论参考。【方法】以水稻受体中花11(ZH11)及其T-DNA插入突变体CF54为材料,利用三引物PCR鉴定出纯合突变株系,并用乙烯利(ET)、水杨酸(SA)和茉莉酸甲酯(MeJA)对ZH11及其纯合突变株系进行处理,测定植株上褐飞虱的蜜露分泌量和虫体增重量。对ZH11及其纯合突变株系的株高、穗长、有效穗数、有效分蘖数、每穗实粒数和千粒重等农艺性状进行测定。【结果】从突变体CF54后代中共筛选出10个株系,其中纯合突变株系有2个,分别为CF54-6和CF54-10。经ET、SA和MeJA处理后ZH11及其纯合突变株系CF54-6和CF54-10植株上褐飞虱的虫体增重量和蜜露分泌量较未处理对照显著(P<0.05,下同)或极显著(P<0.01,下同)下降,但未处理对照间均无显著差异(P>0.05,下同)。经MeJA处理后,纯合突变株系CF54-6和CF54-10植株上褐飞虱的虫体增重量和蜜露分泌量较ZH11显著下降,但经SA和ET处理的纯合突变株系CF54-6和CF54-10植株上褐飞虱的蜜露分泌量和虫体增重量与ZH11无显著差异。纯合突变株系CF54-6和CF54-10的有效分蘖数、有效穗数和千粒重与ZH11无显著差异,但株高、穗长和每穗实粒数显著或极显著高于ZH11。【结论】ET、SA和MeJA 3种外源生长激素均能诱导水稻野生型及其纯合突变株系植株对褐飞虱的取食与生长发育产生抑制作用,尤其是MeJA抑制效果最佳,推测纯合突变株系抗虫性受茉莉酸(JA)信号途径的影响更大。T-DNA插入明显影响水稻植株的农艺性状。
【Objective】The purpose was to identify the effect of growth hormone treatment on brown planthopper(BPH)resistance of rice T-DNA insertion mutants,and to detect the agronomic traits of the mutants,which could provide theoretical reference for the study of molecular mechanism of insect resistance mediated by mutant gene.【Method】Rice variety Zhonghua 11(ZH11)and its T-DNA insertion mutant CF54 were used as materials.Homozygous insertion mutants were identified by the tri-primers PCR method.ZH11 and its homozygous mutant were treated with ethephon(ET),salicylic acid(SA)and methyl jasmonate(MeJA)to measure the BPH honeydew secretion and the weight gain.Finally,agronomic traits,such as the plant height,spike length,effective spikes,number of productive panicles,grain number per spike and 1000-grain weight of ZH11 and the mutants were measured.【Result】Ten lines were screened out from the offspring of mutant CF54,including two homozygous mutant lines CF54-6 and CF54-10.After ET,SA or MeJA treatments,the body weight gain and honeydew secretion of ZH11 and its homozygous mutant strains CF54-6 and CF54-10 on plants were significantly(P<0.05,the same below)or extremely(P<0.01,the same below)lower than those of untreated control,but no significant difference was detected between untreated controls(P>0.05,the same below).After treated with MeJA,the homozygous mutant lines CF54-6 and CF54-10 displayed significant reduction in BPH honeydew secretion and the weight gain comparing with ZH11.However,there was no significant difference in honeydew secretion and body weight gain between homozygous mutants CF54-6 and CF54-10 treated with SA and ET and ZH11.The productive panicles,effective spikes and 1000-grain weight of homozygous mutant lines CF54-6 and CF54-10 treated by SA and ET were not significantly different from those of ZH11.But the plant height,spike length,and grain number per spike increased significantly or extremely higher than ZH11.【Conclusion】The three exogenous growth hormones ET,SA and MeJA can induc
作者
舒宛
王心怡
李志华
李洋
何勇
邱永福
田志宏
SHU Wan;WANG Xin-yi;LI Zhi-hua;LI Yang;HE Yong;QIU Yong-fu;TIAN Zhi-hong(College of Life Science,Yangtze University,Jingzhou,Hubei 434025;College of Agriculture,Guangxi University,Nanning 530004,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2019年第2期230-236,共7页
Journal of Southern Agriculture
基金
国家自然科学基金项目(31560423)
广西自然科学基金项目(2013GXNSFGA019009)
