摘要
目的探讨滤泡辅助性T淋巴细胞(follicular helper T lymphocyte,Tfh)细胞及其转录因子无刚毛鳞甲复合体样蛋白2(achaete-scute homologue 2,Ascl2)在慢性HBV感染中的表达和意义。方法选择CHB患者40例,健康对照者30名。采用流式细胞术检测CD4^+CXCR5^+Tfh细胞及CD4^+CXCR5^+PD-1^+Tfh细胞频数;实时定量PCR法检测Tfh细胞中Ascl2表达水平;ELISA检测血清中各细胞因子水平。结果与健康对照者相比:CHB患者的CD4^+CXCR5^+Tfh及CD4^+CXCR5^+PD-1^+Tfh细胞频数升高,差异有统计学意义(t=4.786,P<0.05);CHB患者中Ascl2mRNA表达升高,差异有统计学意义(t=4.655,P<0.05);CHB患者的IL-21、IL-17均升高,差异均有统计学意义(t=4.896和t=3.764,P<0.05)。结论 Ascl2可能与HBV感染者Tfh细胞的分化发育有关。
Objective To investigate the significance of achaete-scute homologue 2(Ascl2)expression in T follicular helper(Tfh)cells during chronic hepatitis B virus infection.Methods Frequencies of CD4+CXCR5+Tfh cells and CD4+CXCR5+PD-1+Tfh cells in peripheral blood mononuclear cells(PBMC)were analyzed by flow cytometry,respectively.The expression of Ascl2 in Tfh cells was determined by quantitative real-time polymerase chain reaction.The levels of serum interleukin-21(IL-21)and interleukin-17(IL-17)were measured using enzyme-linked immunosorbent assay.Results The frequencies of CD4+CXCR5+and CD4+CXCR5+PD-1+Tfh cells were significantly higher in PBMC from chronic hepatitis B(CHB)patients than that from healthy controls(t=4.786,P<0.05).The expression of Ascl2 was significantly higher in Tfh cells of CHB patients(t=4.655,P<0.05).Furthermore,the levels of serum IL-21 and IL-17 were significantly higher in CHB patients(t=4.896 and t=3.764,P<0.05).Conclusion It was implied that transcription factor Ascl2 plays an important role in the differentiation and development of Tfh cells in CHB patients.
作者
王亚宁
范超
申焕君
郝春秋
贾战生
WANG Ya-ning;FAN Chao;SHEN Huan-jun;HAO Chun-qiu;JIA Zhan-shen(Department of Infectious Diseases,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,China)
出处
《肝脏》
2018年第12期1069-1072,共4页
Chinese Hepatology
基金
国家自然科学基金资助项目(81471541)
