摘要
【目的】探讨^(18)F-FDG PET/CT显像评价SD大鼠肝纤维化疾病。【方法】12只(170±10)g雄性SD大鼠随机分为模型组(TAA组)和对照组,每组6只。模型组大鼠每周两次腹腔注射4%硫代乙酰胺,每次剂量200 mg/kg;对照组腹腔注射等比例生理盐水。注射6周后对两组大鼠行^(18)F-FDG PET/CT显像,检测肝组织摄取^(18)F-FDG情况。显像后解剖两组大鼠观察肝脏解剖形态学变化并取其肝脏用于HE染色、Masson染色和羟脯氨酸含量测定。【结果】模型组解剖形态显示肝脏肿胀、表面欠光滑、边缘圆钝,对照组肝脏大小及比例正常、外表光滑、边缘锐利;HE染色显示模型组大鼠肝血窦区和肝细胞周围中性粒细胞大量募集;Masson染色显示模型组大鼠肝组织明显纤维增生和胶原沉积;羟脯氨酸含量测定显示模型组大鼠肝组织羟脯氨酸含量增高(P<0.001);^(18)F-FDG PET/CT显像模型组大鼠肝脏放射性摄取增加,其^(18)F-FDG摄取值明显高于对照组(P<0.001)。【结论】^(18)F-FDG PET/CT显像可用于无创诊断肝纤维化。
【Objective】To assess the value of 18F-FDG PET/CT for imaging liver fibrosis.【Methods】12 male SD rats(170±10 g)were divided into model group(TAA group)and control group.In the model group,200 mg/kg thioacetamide dissolved in sterile saline was administered to rats by means of intraperitoneal injection twice a week for a total of 6 weeks.In the control group,rats were treated with the same volume of saline.At week 6 after injection,18F-FDG PET/CT imaging was performed on two groups and measurement of the liver 18F-FDG uptake in two groups was taken.After PET/CT scans,all rats were sacrificed to observe anatomical morphological changes.Liver tissues were harvested for hematoxylin and eosin(H&E)staining,Masson staining and measurement of hydroxyproline levels.【Results】Liver anatomical morphology of the TAA-induced rats was roughness with brunt margins and coarse surfaces,while control rats showed sharp margins and smooth surfaces.HE staining showed visible histological changes that hepatocyte and liver sinus area surrounded by plentiful recruited hepatocyte neutrophils in the model group.Masson staining showed that obvious proliferation of fibroblasts and deposition of collagen in liver tissues in the model group.Model group showed higher hydroxyproline content than that in the control group(P<0.001).The results of 18F-FDG imaging indicated that apparent liver radioactivity concentration in the model group.18F-FDG uptake value of liver tissues in the model group was higher than that in the control group(P<0.001).【Conclusion】18F-FDG PET/CT imaging could be used for diagnosis of liver fibrosis noninvasively.
作者
苏舒
唐刚华
聂大红
熊鹦
马慧
林丽萍
元龚骏
向贤宏
SU Shu;TANG Gang-hua;NIE Da-hong;XIONG Ying;MA Hui;LIN Li-ping;YUAN Gong-jun;XIANG Xian-hong(Department of Imaging and Nuclear Medicine,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080)
出处
《中山大学学报（医学版）》
CAS
CSCD
北大核心
2019年第1期124-129,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(81671719)
广东省科技计划项目(2016B090920087
2013B021800264)
广州市科技计划项目(201604020169
201510010145)
广东省医学科学基金(2015A030313067)
