摘要
目的探讨人参对感染后小鼠肠黏膜的干预效果及对血清C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平的影响。方法 60只小鼠中,随机抽取15只为空白对照组,剩余45只用于制备重症腹腔感染模型(感染组)。然后再将45只小鼠模型采用随机数字表法分为三组:模型组、抗炎组、抗炎+人参组,每组各15只。抗炎组给予亚胺培南/西司他丁333.33 mg/kg(腹腔注射,12 h/次),抗炎+人参组在抗炎组的基础上给予人参皂苷Rb1(纯度98%以上)50 mg/(kg·d)灌胃,模型组给予等量无菌生理盐水腹腔注射。分别于治疗后24、48、72 h取血标本,每组每次5只,观察各组小鼠肠黏膜CRP、TNF-α、IL-6水平变化情况;留取末端回肠,行HE染色,观察肠黏膜层情况,并计算肠黏膜上皮细胞凋亡指数。结果重症腹腔感染后,小鼠肠黏膜出现萎缩,肠黏膜上皮细胞凋亡数目明显增多,且肠黏膜萎缩程度越明显,肠黏膜上皮细胞凋亡数目越多。治疗后72 h,抗炎+人参组重症腹腔感染小鼠的CRP、TNF-α、IL-6水平显著降低(P <0.05或P <0.01),且下降幅度大于抗炎组(P <0.05);抗炎+人参组细胞凋亡指数显著低于模型组和抗炎组(P <0.05或P <0.01)。结论重症腹腔感染可导致肠黏膜萎缩,肠黏膜萎缩与肠黏膜上皮细胞凋亡数目有关;人参对肠黏膜有保护作用,其能改善肠黏膜萎缩的症状,减少对肠黏膜分泌物的影响。
Objective To investigate the effects of ginseng on intestinal mucosa and the levels of serum C-reactive protein(CRP),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in mice after infection.Methods Among 60 mice,15 mice were randomly selected as blank control group,and the remaining 45 mice were used to prepare severe abdominal infection model(infected group).Then 45 mice models were divided into three groups by the random number table method:model group,anti-inflammatory group,anti-inflammatory+ginseng group,with 15 mice in each group.The anti-inflammatory group was given Imipenem/Cilastatin 333.33 mg/kg(intraperitoneal injection,once 12 h);the anti-inflammatory+ginseng group was given intragastric administration of ginsenoside Rb1(purity >98%)50 mg/(kg·d)on the basis of the anti-inflammatory group;the model group was given the same amount of sterile saline by intraperitoneal injection.Blood samples were taken at 24,48 h and 72 h after treatment.Five rats in each group were used to observe the changes of CRP,TNF-αand IL-6 levels in the intestinal mucosa of mice in each group;the terminal ileum was harvested and HE staining was performed.The intestinal mucosal layer was observed and the intestinal epithelial cell apoptosis index was calculated.Results After severe intraperitoneal infection,mice intestinal mucosal atrophied,intestinal epithelial cell apoptosis increased significantly,and the more obvious the intestinal mucosal atrophied,the greater the number of intestinal mucosal epithelial cell apoptosis was.After treatment for 72 h,the levels of CRP,TNF-αand IL-6 in mice with severe intraperitoneal infection of anti-inflammatory+ginseng group were significantly decreased(P<0.05 or P<0.01),and the decrease range was greater than that of anti-inflammatory group(P<0.05);the apoptotic index of anti-inflammatory+ginseng group was significantly lower than that of the model group and the anti-inflammatory group(P<0.05 or P<0.01).Conclusion Severe abdominal infection can cause intestinal mucosal atrophy,intest
作者
冯其麟
邓艾平
刘珏
范彦博
FENG Qilin;DENG Aiping;LIU Jue;FAN Yanbo(Department of Pharmacy,Wuhan Central Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Province,Wuhan 430014,China;Department of Scientific Research,Wuhan Hospital of Traditional Chinese Medicine,Hubei Province,Wuhan 430014,China)
出处
《中国医药导报》
CAS
2019年第4期18-21,共4页
China Medical Herald
基金
湖北省武汉市卫计委医疗卫生科研项目(WZ14Z01)
关键词
腹腔感染
肠黏膜
人参
细胞凋亡
Intraperitoneal infection
Intestinal mucosa
Ginseng
Cell apoptosis
