摘要
目的探讨冠心病患者血浆miR-92a的表达水平及其调控通路。方法选取自2017年10月至2018年2月就诊于北部战区总医院的118例胸痛患者为研究对象。其中,对照组35例,冠心病组83例[不稳定性心绞痛(UA)亚组34例,急性心肌梗死(AMI)亚组49例]。检测受试者血浆中miR-92a的相对表达量;使用miRNA数据库预测相关靶基因;通过GO分析和KEGG通路分析预测其与冠心病相关的信号通路。检测受试者血小板聚集率,判断是否存在阿司匹林抵抗(AR)及氯吡格雷抵抗(CR)。结果对照组血浆中miR-92a水平明显高于UA亚组和AMI亚组(P <0. 05); UA亚组与AMI亚组miR-92a水平比较,差异无统计学意义(P> 0. 05)。通过数据库预测,共有的miR-92a靶基因118个。GO分析及KEGG通路分析显示,与冠心病相关最显著的信号通路为血小板活化。对照组中,AR患者miR-92a水平显著低于非阿司匹林抵抗(NAR)患者(P <0. 05); CR患者miR-92a水平显著低于非氯吡格雷抵抗(NCR)患者(P <0. 05)。冠心病组中,AR患者与NAR患者、CR患者与NCR患者miR-92a水平比较,差异均无统计学意义(P> 0. 05)。结论 miR-92a在非冠心病者外周血中高表达,可能通过抑制血小板活化延缓冠心病进程。
Objective To investigate the expression level of plasma miR-92 a and its possible regulatory pathways in patients with coronary heart disease( CHD). Methods A retrospective study was performed on 118 cases of patients with chest pain who were admitted from October 2017 to February 2018. Patients were divided into the control group,with 35 cases and the CHD group,with 83 cases[34 cases in the unstable angina( UA) subgroup and 49 cases in the acute myocardial infarction( AMI) subgroup]. The relative expression of miR-92 a in the plasma of the subjects was detected. MiRNA database was used to predict related target genes. GO analysis and KEGG pathway analysis were used to predict the signal pathways related to coronary heart disease. The subjects’ platelet aggregation rate was measured to determine the presence of aspirin resistance( AR) and clopidogrel resistance( CR). Results The plasma level of mir-92 a in the control group was significantly higher than that in the UA subgroup and AMI subgroup( P < 0. 05). There was no significant difference in mir-92 a level between UA subgroup and AMI subgroup( P > 0. 05). A total of 118 target genes of mir-92 a were predicted by the database. GO analysis and KEGG pathway analysis showed that the most significant signal pathway related to coronary heart disease was platelet activation. In the control group,the level of mir-92 a in AR patients was significantly lower than that in non-aspirin resistance( NAR) patients( P < 0. 05). Mir-92 a level in CR patients was significantly lower than that in non-clopidogrel resistance( NCR) patients( P < 0. 05). In the CHD group,mir-92 a levels of AR patients and NAR patients,CR patients and NCR patients were compared( P < 0. 05). Conclusion Compared with CHD patients,mir-92 a is highly expressed in peripheral blood of non-CHD patients,which may delay the progression of CHD by inhibiting platelet activation.
作者
刘玉薇
冯晓敏
万楠
LIU Yu-wei;FENG Xiao-min;WAN Nan(Dalian Medical University,Dalian 116044,China)
出处
《临床军医杂志》
CAS
2019年第2期142-145,共4页
Clinical Journal of Medical Officers
关键词
冠心病
miR-92a
血小板活化
血小板聚集率
Coronary heart disease
MiR-92a
Platelet activation
Platelet aggregation rate
