摘要
目的观察样本保存时间对流式细胞仪检测肝癌患者外周血T淋巴细胞亚群的影响。方法随机选取肝癌患者16例,采血后6 h内分离外周血单核细胞,将细胞分成五组,包括立即上机组、4℃PBS保存1 d、2 d、3 d及5 d检测组,分别进行流式检测。结果样本保存3 d组淋巴细胞的物理参数发生变化;样本保存3 d组CD3+T淋巴细胞百分比显著降低(P <0. 05);样本保存3 d组NK细胞百分比显著降低(P <0. 05)。结论临床样本在收集后进行外周血单核细胞分离,将细胞用PBS于4℃条件下保存3 d内进行淋巴细胞和NK细胞的上机检测结果比较稳定。
Objective To study the effect of storage time of samples for cytometry on subsets of T lymphocytes of hepatocellular carcinoma patients. Methods Sixteen patients with hepatocellular carcinoma were collected randomly, and they were processed within 6 hours, then samples were tested by flow cytometry analysis within 1 hour, 1 day, 2 days, 3 days and 5 days, separately. The samples were stored in PBS at 4 ℃. Results At 3 days of samples treatment, the physical parameters of lymphocytes were changed;at 3 days of sample treatment, the percentage of surviving CD3 +T lymphocytes was decreased significantly ( P <0.05). At 3 days of sample treatment, the percentage of NK cells was decreased significantly ( P <0.05). Conclusion The results of T lymphocytes and NK cells of hepatocellular carcinoma patients analyzed by flow cytometry are stable within 3 days after the samples were processed stored in PBS at 4 ℃.
作者
代艳超
覃岭
高梦丹
孙坚萍
张永宏
DAI Yanchao;QIN Ling;GAO Mengdan;SUN Jianping;ZHANG Yonghong(Biomedical Information Center,Beijing You’An Hospital,Capital Medical University,Beijing 100069,China)
出处
《胃肠病学和肝病学杂志》
CAS
2019年第2期167-170,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然基金委员会(81320108017)
北京市科学技术委员会(Z171100001017078)
北京市重点实验室(BZ0373)
首都医科大学附属北京佑安医院中国初级保健基金会(YNKT20160030)
关键词
肝癌
样本保存时间
流式细胞仪
T淋巴细胞
NK细胞
Hepatocellular carcinoma
Storage time of samples
Flow cytometry
T lymphocyte
NK cells
