摘要
目的观察长链非编码RNA分化拮抗非编码RNA(lncRNA DANCR)在结肠癌组织细胞中的表达情况及其对结肠癌细胞侵袭能力的影响。方法采用qRT-PCR法检测92例结肠癌及癌旁组织中的DANCR,比较不同临床病理特征患者结肠癌组织中DANCR表达; qRT-PCR法检测结肠癌细胞LOVO及正常肠上皮细胞NCM460中的DANCR;将LOVO细胞随机分为对照组和沉默组,分别转染对照质粒si-con及DANCR沉默质粒si-DANCR,qRT-PCR法检测DANCR,Transwell侵袭实验计数穿膜细胞数评价细胞侵袭能力,Western blotting法检测侵袭转移相关因子基质金属蛋白酶9(MMP9)。结果结肠癌组织DANCR相对表达量为高于癌旁组织,LOVO细胞DANCR相对表达量高于NCM460细胞(P均<0. 01)。临床分期Ⅲ期+Ⅳ期、T分期T3期+T4期、淋巴结转移和远处转移患者结肠癌组织中DANCR高表达率高于临床分期Ⅰ期+Ⅱ期、T分期T1期+T2期、无淋巴结转移和无远处转移者(P均<0. 01)。与对照组比较,沉默组细胞DANCR表达降低、穿膜细胞减少、细胞MMP9蛋白表达降低(P均<0. 01)。结论 lncRNA DANCR在结肠癌组织及细胞中表达升高,在结肠癌的发生发展过程中起到了癌基因的角色; DANCR可提高结肠癌细胞的侵袭能力,该作用与上调MMP9蛋白表达有关。
Objective To observe expression of long non-coding RNA(lncRNA)differentiation antagonizing non-coding RNA(DANCR)in the colorectal cancer(CRC)cells and to explore its effect on the cell invasion.Methods The expression of DANCR in the CRC tissues and paired para-tumor tissues was determined by qRT-PCR.DANCR expression in the CRC patients with different clinicopathological features was measured.The qRT-PCR was applied to evaluate the expression of DANCR in the CRC cell line LOVO and normal epithelial cell line NCM460.LOVO cells were divided into the control group and DANCR-silenced group(or si-DANCR group)randomly,and were transfected with si-con and si-DANCR,respectively.The expression of DANCR was detected by qRT-PCR.We used the Transwell invasion assay to count the number of transmembrane cells and evaluate cell invasion.Western blotting was applied to detect the expression of invasion and metastasis-related factor matrix metalloproteinase 9(MMP9).Results The expression of DANCR in the CRC tissues was higher than that in the para-tumor tissues.Additionally,the expression of DANCR in the LOVO cells was higher than that in the NCM460 cells(both P<0.01).The expression of DANCR in the CRC patients with advanced clinical staging(stageⅢandⅣ),higher T staging(T 3 and T 4),and distant metastasis was higher than that in CRC patients with early clinical staging(stageⅠandⅡ),lower T staging(T 1 and T 2),and non-distant metastasis(P<0.01).Compared with the control group,the expression of DANCR,the transmembrane cells,and the expression of MMP9 protein decreased in the si-DANCR group(all P<0.01).Conclusion LncRNA DANCR is up-regulated in CRC tissues and cells,and DANCR works as an oncogene in the progression of CRC;DANCR promotes the metastatic ability of CRC cells,which is related to the up-regulation of MMP9.
作者
李涛
王江
王舒
朱玄
LI Tao;WANG Jiang;WANG Shu;ZHU Xuan(The Seventh Clinical College of China Medical University,General Hospital of Fushun Mining Bureau, Fushun 113008,China)
出处
《山东医药》
CAS
2019年第5期29-32,共4页
Shandong Medical Journal
关键词
结肠癌
长链非编码RNA
分化拮抗非编码RNA
肿瘤侵袭
colon carcinoma
long non-coding RNA
differentiation antagonizing non-coding RNA(DANCR)
tumor invasion
