摘要
针对水产品中隐性孔雀石绿(leuco malachite green,LMG)残留的问题,建立了鱼肉中LMG的分子印迹仿生酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测方法。以LMG为模板分子,在p H=8. 5的Tris-HCl介质中,多巴胺(dopamine,DA)经氧化自聚合4 h即可在微孔板表面形成聚合物膜,经V(甲醇)∶V(乙酸)=9∶1混合溶液洗脱模板分子后,形成LMG分子印迹聚合物膜(membrane of molecularly imprinted polymer,MIP)。以LMG-MIP膜为仿生抗体,建立了鱼肉中LMG残留的仿生ELISA检测方法。该方法灵敏度为5. 18μg/L,检出限达0. 03μg/L。利用隐性结晶紫和孔雀石绿这2种LMG的结构类似物进行方法的特异性试验,交叉反应率为21. 3%。实际样品加标回收实验表明,方法的回收率为71. 8%～73. 5%,RSD≤4. 2%。结果表明,该检测方法可用于鱼肉中LMG残留的快速筛查。
For the detection of leuco malachite green(LMG)in aquatic products,a biomimetic ELISA method was established based on the biomimetic antibody membrane of molecularly imprinted polymer(MIP).Using LMG as template molecules,the LMG-MIP membrane could be formed on the surface of microplates through the dopamine(DA)self-polymerization for 4 h in Tris-HCl(pH=8.5).After elution of LMG by methanol-acetic acid at the volume ratio of 9∶1,the LMG-MIP membrane on microplates could be used as biomimetic antibody of LMG in ELISA.The sensitivity estimated with IC50 was 5.18μg/L and the detection limit was 0.03μg/L.The cross-reactivities for two structural analogues of LMG were 21.3%for malachite green.The recovery tested by LMG standard spiking in fish samples ranged from 71.8%to 73.5%,and the average relative standard deviation was less than 4.2%.The results showed that the method could be used for the rapid detection of LMG residues in fish.
作者
彭爱红
王茜雅
林郑忠
陈晓梅
黄志勇
PENG Aihong;WANG Xiya;LIN Zhengzhong;CHEN Xiaomei;HUANG Zhiyong(College of Food and Biological Engineering,Jimei University,Xiamen 361021,China)
出处
《集美大学学报（自然科学版）》
CAS
2019年第1期14-19,共6页
Journal of Jimei University：Natural Science
基金
福建省自然科学基金项目(2015J01615)
福建省科技计划项目(2016Y0064)
福建省海洋功能食品工程中心开放课题(C11172)
集美大学创新团队基金项目(2010A007)
