摘要
目的探讨2型糖尿病患者血清分泌型Klotho(sKlotho)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、单核趋化蛋白1(MCP-1)、转化生长因子β1(TGF-β1)表达水平及意义。方法将252例2型糖尿病患者根据尿白蛋白/肌酐比率(UACR)分为正常尿白蛋白组(UACR <30 mg/g)、微量尿白蛋白组(30 mg/g≤UACR <300 mg/g)和大量尿白蛋白组(UACR≥300 mg/g);另选同期100例身体健康者为健康对照组。收集受试者尿白蛋白、血尿肌酐,估算肾小球滤过率(eGFR)、糖化血红蛋白(HbA1c)、空腹血糖(FPG)、口服糖耐量试验2 h血糖(2 h PG)、胰岛素抵抗指数(HOMA-IR)、总胆固醇(TC)以及三酰甘油(TG)指标;酶联免疫(ELISA)检测血清sKlotho、NGAL、MCP-1、TGF-β1水平。结果 2型糖尿病患者的收缩压较健康对照组显著升高(P <0. 05);正常尿白蛋白组和大量尿白蛋白组的舒张压较健康对照组显著升高(P <0. 05)。2型糖尿病患者的eGFR、HbA1c、FPG、2 h PG、TC、TG水平随病情的加重逐渐增加(P <0. 05)。ELISA结果显示,2型糖尿病患者sKlotho水平随病情的加重逐渐降低(P <0. 05),NGAL、MCP-1、TGF-β1水平随病情加重逐渐增加(P <0. 05)。2型糖尿病患者血清sKlotho水平与UACR、HbA1c、FPG、2 h PG、HOMA-IR、TC、TG呈负相关(P <0. 05),与e GFR呈正相关(P <0. 05);血清NGAL、MCP-1、TGF-β1水平与体重指数、UACR、HbA1c、FPG、2 h PG、HOMA-IR、TC、TG呈正相关(P <0. 05),与eGFR呈负相关(P <0. 05)。结论血清s Klotho、NGAL、MCP-1、TGF-β1水平可能成为2型糖尿病的早期诊断标志物,其机制可能与炎性反应、肾脏损伤等有关。
Objective To explore the expression and significance of serum secreting type sKlotho(sKlotho),neutrophil gelatinase-associated lipocalin(NGAL),monocyte chemoattractant protein 1(MCP-1)and transforming growth factorβ1(TGF-β1)in patients with type 2 diabetes mellitus(T2DM).Methods A total of 252 patients with T2DM were divided into normal urine albumin group(UACR<30mg/g),microalbuminuria group(30mg/g<UACR<300mg/g)and large urinary albumin groups(UACR >300mg/g)according to the urinary albumin/creatinine ratio(UACR),at the same time,the other 100 healthy subjects were enrolled as control group.The levels of urinary albumin,hematuria creatinine,estimated glomerular filtration rate(eGFR),glycosylated hemoglobin(HbA1c),fasting blood glucose(FPG),oral glucose tolerance test blood glucose 2h(2h PG)and insulin resistance index(HOMA-IR),total cholesterol(TC)and trigalloyl glycerol(TG)were detected and compared among groups.In addition,enzyme linked immunosorbent assay(ELISA)was used to detect the serum levels of sKlotho,NGAL,MCP-1 and TGF-β1.Results The systolic blood pressure in patients with T2DM was significantly higher than that in control group(P<0.05).The levels of diastolic blood pressure of patients in normal urine albumin group and large urine albumin group were significantly higher than those in control group(P<0.05).Moreover,the levels of eGFR,HbA1c,FPG,2 h PG,TC,TG,NGAL,MCP1 and TGF-β1 of patients with T2DM were increased gradually,and the levels of sKlotho were decreased gradually with the aggravation of disease(P<0.05).In addition,the serum levels of sKlotho were negatively correlated with those of UACR,HbA1c,FPG,2h PG,HOMAIR,TC and TG(P<0.05),however,which were positively correlated with those of eGFR(P<0.05).The serum levels of NGAL,MCP1 and TGF-β1 were positively correlated with body mass index,UACR,HbA1c,FPG,2h PG,HOMAIR,TC and TG(P<0.05),however,which were negatively correlated with eGFR(P<0.05).Conclusion The serum levels of sKlotho,NGAL,MCP1 and TGF-β1 may be regarded as the markers of early diagn
作者
刘洪
冯小兰
袁晓玲
尤鸶
LIU Hong;FENG Xiaolan;YUAN Xiaoling(Department of Endocrinology,Sichuan Academgorodok Hospital,Sichuan,Mianyang 621900,China)
出处
《河北医药》
CAS
2019年第2期186-189,共4页
Hebei Medical Journal
基金
四川省卫生厅科研基金项目(编号:150143)
