摘要
目的:探讨慢性乙型肝炎患者外周血中B7-H3变化趋势。方法:收集广西中医药大学第一附属医院2015年5月至2016年6月肝病科患者血清样本共47例,根据其乙肝两对半结果而分为4组,即大三阳组、小三阳组、慢性乙型肝炎组、肝纤维化组,并从广西中医药大学第一附属医院体检中心随机收取10份HBsAg阴性健康人血清样本作为对照组。用ELISA方法检测其血清样本中B7-H3含量,并两两比较各组之间的差异;用RT-PCR的方法检测患者组样本血清中HBV DNA拷贝数,并分析其与B7-H3的相关性。结果:对照组血清样本中B7-H3含量为(7.36±0.36)ng/mL,高于另外4组患者组,差异有统计学意义(P<0.05);患者组4组B7-H3含量均为(2.12～2.81)ng/mL之间,4组间两两比较均无统计学意义(P>0.05);4组患者组HBV DNA检测结果显示,大三阳组HBV DNA拷贝数达到(602.2±193.2)×105/m L,与肝纤维化组相比无统计学差异(P>0.05);大三阳组、肝纤维化组患者HBV DNA拷贝数与小三阳组、慢性HBV感染组相比,其拷贝数明显升高,差异有统计学意义(P<0.05);所有患者组样本中B7-H3含量与HBVDNA拷贝数相关性分析结果显示,二者无明显相关性(P>0.05)。结论:乙型肝炎患者外周血中B7-H3含量与正常人相比有所下降,可能与B7-H3在患者体内重新分布有关,与乙型肝炎病毒的活跃程度无明显相关性。
Objective:To investigate the changes of B7-H3 in peripheral blood of patients with chronic hepatitis B.Methods:Atotal of 47 serum samples from patients with hepatic diseases fromMay 2015 to June 2016 were collected.According to the results of detection of hepatitis B,they were divided into 4 groups,namely,Da-san-yang group,Xiao-san-yang group and chronic hepatitis B group,and liver fibrosis group,respectively.Ten HBsAg-negative healthy human serum samples collected from the First Affiliated Hospital of Guangxi University of Chinese Medicine were as the control group.The B7-H3 content in the serum samples were measured by ELISA,and the differences between each two groups were compared by SPSS.Results:The B7-H3 content in the serum samples of the control group was(7.36±0.36)ng/mL,which was higher than that of the other 4 groups(P<0.05).The B7-H3 contents of the patients in the 4 groups were the range of 2.12ng/mL and 2.81ng/ml,and there was no significant difference between each two groups(P >0.05).The results of RT-PCR assay of the four groups showed that the copies of HBV DNA in the Da-san-yang group reached(602.2±193.2)×10^5/mL,which was not significantly different from the liver fibrosis group(P >0.05).The copies of HBV DNA in the patients of Da-san-yang group and liver fibrosis group were significantly higher than that in Xiaosanyang group and chronic HBV infection group,the difference was statistically significant(P<0.05).The result of correlation analysis between B7-H3 content and HBV DNAcopy number in all patient samples showed that there was no significant correlation between the two factors(P >0.05).Conclusion:The content of B7-H3 in peripheral blood of patients with hepatitis B is lower than that of normal people,which seems to be related to the redistribution of B7-H3 in the body of patient,and there were no significant correlation between the content of B7-H3 and the activity of hepatitis B virus.
出处
《大众科技》
2019年第1期20-22,29,共4页
Popular Science & Technology
基金
广西壮族自治区教育厅"广西高校科研项目"(YB2014170)
广西中医药大学第一附属医院青年基金(2016QN015)
