摘要
目的人工诱变选育高产四氢嘧啶的菌株。方法以海神盐单胞菌Halomonas neptunia ATCC BAA-805为出发菌株,采用紫外线和亚硝基胍复合诱变处理,获得四氢嘧啶产量提高突变株,采用单因素实验优化发酵培养基及发酵条件,进一步提高突变株的四氢嘧啶产量。结果 Halomonas neptunia ATCC BAA-805经复合诱变处理,获得突变株UN-2,摇瓶发酵四氢嘧啶产量达1.8 g/L,与出发菌株相比产量提高了53.8%。单因素优化实验结果为UN-2菌株在酵母粉浓度4.0 g/L,葡萄糖浓度20 g/L,氯化钠浓度7%(w/v),p H 7.5条件下,四氢嘧啶产量为5.53 g/L。结论本研究诱变选育的菌种是适于商业化生产四氢嘧啶的优良菌株。
Objective To screen the ectoine high-productive strain by induced mutation. Methods Halomonas neptunia ATCC BAA-805 was treated by ultraviolet radiation and nitrosoguanidine culturing to get mutants with higher yields of ectoine. The ectoine yield of the mutants was further increaesd by optimization of the fermentation culture medium and condition through single factor experiment. Results The mutant Halomonas neptunia UN-2 with high-yield of ectoine was obtained by compound mutagenesis. The yield of ectoine by shaking flask fermentation was 1.8 g/L, which was 53.8 % higher than that of the original strain. The result of single factor experiment showed that the ectoine yield of the strain UN-2 could reach 5.53 g/L under the optimal conditions: yeast powder 4.0 g/L , glucose of 20 g/ L, NaCl of 7 %(w/v), and pH of 7.5. Conclusion The mutagenic strain screened in this study is an excellent strain suitable for commercial production of ectoine.
作者
王冠凤
石艳丽
钱晓路
董艳美
陈晨
栾贻宏
郭学平
WANG Guan-feng;SHI Yan-li;QIAN Xiao-lu;DONG Yan-mei;CHEN Chen;LUAN Yi-hong;GUO Xue-ping(Bloomage Freda Biopharm Co., Ltd., Jinan 250100, China)
出处
《食品与药品》
CAS
2019年第1期49-56,共8页
Food and Drug
关键词
紫外线
亚硝基胍
海神盐单胞菌
四氢嘧啶
优化
ultraviolet
nitrosoguanidine
Halomonas neptunia
ectoin
optimization
